نتایج جستجو برای: recombinant momp protein

تعداد نتایج: 1297357  

Journal: :Journal of cell science 2013
Jisen Huai F-Nora Vögtle Lars Jöckel Yunbo Li Thomas Kiefer Jean-Ehrland Ricci Christoph Borner

When NF-κB activation or protein synthesis is inhibited, tumor necrosis factor alpha (TNFα) can induce apoptosis through Bax- and Bak-mediated mitochondrial outer membrane permeabilization (MOMP) leading to caspase-3 activation. Additionally, previous studies have implicated lysosomal membrane permeability (LMP) and formation of reactive oxygen species (ROS) as early steps of TNFα-induced apopt...

Journal: :The Journal of infectious diseases 2010
Gerald I Byrne

An unanswered question concerning prevalence and disease severity of Chlamydia trachomatis genital infection is whether more prevalent strains or strains more likely to cause serious disease complications are causally associated with specific virulence attributes. The major method for distinguishing chlamydial strains is based on differences in the major outer membrane protein (MOMP). A subset ...

Background and Aims: Q59L mutant of L-asparaginase enzyme from Escherichia coli (E. coli) has been introduced with lower side effects. This version of the enzyme might have potential applications in the treatment of leukemia patients. We utilized SHuffle T7 strain of E. coli, to produce the mutant enzyme in the presence of chaperone molecules. Materials and Methods: Q59LAsp gene was cloned in...

Journal: :iranian journal of public health 0
a karami p hindersson n høiby s morovvati

lyme borreliosis is a tick-born disease caused by the spirochete borrelia burgdorferi. the aim of this research was molecular typing of different strains of b. burgdorferi and protective efficacy of outer surface protein in animal model. seven strains of b. burgdorferi isolated from skin and csf of lyme disease patients were compared using pcr- rflp as well as dna and amino acid sequences. the ...

Chicken infectious bronchitis virus (IBV) causes severe respiratory and renal dysfunction in chickens. In the present study, we used reverse transcription-polymerase chain reaction (RT-PCR) to amplify the nucleocapsid (N) protein gene of strain Massachusetts (Mass) H120 of IBV that is commonly used in the vaccine production in Iran. The PCR product with the expected size of 1.2 kb was cloned in...

Journal: :iranian journal of blood and cancer 0
mehryar habibi roudkenar mahshid mohammadipour arezou oodi raheleh halabian naser amirizadeh nasser masrouri

background: the rh antigens are expressed as parts of a protein complex in the rbc membrane. this complex is a tetramer, consisting of two molecules of rhag and two molecules of rh proteins. to express rhd in rbc membrane, expression of rhag is essential. this co-expression only occurs in the erythroid lineage. k562 cell line has an erythroid lineage. materials and methods: cord blood was used ...

Journal: :iranian journal of biotechnology 2008
seyed abbas shojaosadati seyedeh marjan varedi kolaei valiollah babaeipour amir mohammad farnoud

this paper reviews recent strategies used for increasing specific yield and productivity in high cell density cultures. high cell density cultures offer an efficient means for the economical production of recombinant proteins. however, there are still some challenges associated with high cell density cultivation (hcdc) techniques. a variety of strategies in several aspects including host design...

2017
Ledan Wang Yiqi Cai Yirong Xiong Wangqi Du Danwei Cen Chanqiong Zhang Yiling Song Shanli Zhu Xiangyang Xue Lifang Zhang

Chlamydia trachomatis (Ct) is one of the most frequently encountered sexual infection all over the world, yielding tremendous reproductive problems (e.g. infertility and ectopic pregnancy) in the women. This work described the design of a plasmid vaccine that protect mice from Ct infection, and reduce productive tract damage by generating effective antibody and cytotoxic T cell immunity. The va...

2013
Francis O. Atanu Ernesto Oviedo-Orta Kimberly A. Watson

Major outer membrane proteins (MOMPs) of Gram negative bacteria are one of the most intensively studied membrane proteins. MOMPs are essential for maintaining the structural integrity of bacterial outer membranes and in adaptation of parasites to their hosts. There is evidence to suggest a role for purified MOMP from Chlamydophila pneumoniae and corresponding MOMP-derived peptides in immune-mod...

Journal: :iranian journal of basic medical sciences 0
roghayeh rahimi department of immunology, faculty of medical sciences, tarbiat modares university, tehran , iran massoumeh ebtekar department of immunology, faculty of medical sciences, tarbiat modares university, tehran , iran seyed mohammad moazzeni department of immunology, faculty of medical sciences, tarbiat modares university, tehran , iran ali mostafaie biotechnology research center, kermanshah university, kermanshah, iran mehdi mahdavi department of virology, pasteur institute of iran, tehran, iran

objective(s):multi-epitopic protein vaccines and direction of vaccine delivery to dendritic cells (dcs) are promising approaches for enhancing immune responses against mutable pathogens. escherichia coli is current host for expression of recombinant proteins, and it is important to optimize expression condition. the aim of this study was the optimization of multi-epitopic hiv-1 tat/pol/gag/env ...

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