objective: organophosphorus (ops) compounds are widely used in many pesticides, insecticides and chemical nerve agents. these compounds are hazardous for humans and the environment. organophosphate hydrolase (oph) is a homodimeric protein initially isolated from pseudomonas diminuta mg and flavobacterium species. this enzyme is able to degrade a broad spectrum of toxic ops compounds. using immo...

background: botulinum neurotoxin (bont) complexes consist of neurotoxin and neurotoxin-associated proteins. hemagglutinin-33 (ha-33) is a member of bont type a (bont/a) complex. considering the protective role of ha-33 in preservation of bont/a in gastrointestinal harsh conditions and also its adjuvant role, recombinant production of this protein is favorable. thus in this study, ha-33 was expr...

objective: the incidence of breast cancer is approximately one million which makes this cancer one of the most common among women worldwide. breast cancer comprises 7% of the total death rate caused by cancers. several strategies that use tumor-associated antigen (taa) vaccination and early detection of breast cancer are clinically being developed. breast cancer is caused by increased over expr...

An amalgamation of microbiology, biocatalysis, recombinant molecular biology, and nanotechnology is crucial for groundbreaking innovation in developing nano-biomedicines sensoristics. Enzyme-based nano-biosensor finds prospective applications various sectors (environmental, pharmaceutical, food, biorefineries). These demand reliable catalytic efficiency functionality the enzyme under an extreme...

Mucopolysaccharidoses (MPSs) are lysosomal storage diseases caused by a deficit in the enzymes needed for glycosaminoglycan (GAG) degradation. Enzyme replacement therapy with recombinant human alpha-L-iduronidase successfully reduces lysosomal storage in canines and humans with iduronidase-deficient MPS I, but therapy usually also induces antibodies specific for the recombinant enzyme that coul...

Because animals are not able to synthesize retinoids de novo, ultimately they must derive them from dietary provitamin A carotenoids through a process known as carotene cleavage. The enzyme responsible for catalyzing carotene cleavage ( -carotene 15,15 -dioxygenase) has been characterized primarily in rat intestinal scrapings. Using a recently reported cDNA sequence for a carotene cleavage enzy...

Cellulase is a family of at least three groups of enzymes that participate in the sequential hydrolysis of cellulose. Recombinant expression of cellulases might allow reducing their production times and increasing the low proteins concentrations obtained with filamentous fungi. In this study, we describe the production of Trichoderma reesei cellobiohydrolase II (CBHII) in a native strain of Wic...

We have achieved, to our knowledge, the first high-level heterologous expression of the gene encoding D-ribulose-5-phosphate 3-epimerase from any source, thereby permitting isolation and characterization of the epimerase as found in photosynthetic organisms. The extremely labile recombinant spinach (Spinacia oleracea L.) enzyme was stabilized by DL-alpha-glycerophosphate or ethanol and destabil...