epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant dna techniques. the aim of this study was to sub-clone the peroxisomal protein (pep) cdna into a mammalian expression vector leading to the formation of a  chimeric pep-cdna containing the flag epitope. the flag-pep recombinant cdna was construc...

Nine practical exercises demonstrate the basic principles in recombinant DNA. The exercises explain the principles that DNA equals genes and that changes in DNA cause changes in genetic properties. The aim is to provide a teaching resource that can be used to illustrate the theory and applications of molecular biology to highschool students, undergraduate students, medics, dentists, doctors, nu...

Page Introduction 2 Structures of genes and mRNA molecules 2 Structure of genes 2 Sequences of promoters and enhancers 3 Signals for terminating RNA synthesis 5 Structure of mRNA molecules 5 Structure of genes coding for reproductive polypeptide hormones and hypothalamic releasing factors 7 Structure of gonadotrophin genes 7 Structure of placental lactogen, prolactin and growth hormone genes 10...

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