background: the over-expression of recombinant proteins in large amount is important for production of therapeutic proteins and structural study. there are several systems for expression of recombinant proteins. one of the most relevant expression systems is escherichia coli (e. coli). although this organism has many advantages, most of recombinant proteins expressed in e. coli hosts form inclu...

background: saccharomyces boulardii (s. boulardii) is the best known probiotic yeast. the genetic engineering of this probiotic strain requires the availability of appropriate mutants to accept various gene constructs carrying different selection markers. as the auxotrophy selection markers are under focus, we have generated a ura3 auxotroph mutant of s. boulardii for use in further genetic man...

The University of Michigan Comprehensive Cancer Center Informatics Core, in cooperation with Recombinant Data Corp, has implemented the open-source tranSMART application (http://www.transmartproject.org/) to support data management and analysis for the Colorectal Transdisciplinary Study (CORECT) Consortium coordinating center. CORECT is a post-GWAS consortium study of the genetic and epidemiolo...

Jing Cheng1, Junbing Jiang1, Junxing Zhao1, Zhirui Wang2,3, Yaogui Sun1, Haili Ma1, Kuohai Fan1, Wei Yin1, Na Sun1, Zhiwei Wang1, Xin Zhao1, and Hongquan Li1* College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China Transplantation Biology Research Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02148, USA MGH-DF/HCC Re...

Tridentate chelate ligands of 2,6-bis[hydroxy(methyl)amino]-1,3,5-triazine family rapidly release iron from human recombinant ferritin in the presence of oxygen. The reaction is inhibited by superoxide dismutase, catalase, mannitol and urea. Suggested reaction mechanism involves reduction of the ferritin iron core by superoxide anion, diffusion of iron(II) cations outside the ferritin shell, an...

The cell-free protein synthesis (CFPS) system, as a technical core of synthetic biology, can simulate the transcription and translation process in an vitro open environment without complete living cell. It has been widely used basic applied research fields because its advanced engineering features flexibility controllability. Compared to typical crude extract-based CFPS due defined customizable...

conclusion: the newly produced recombinant protein bmk revealed to be immunogenic. moreover, anti-bmk antibodies produced in mice were able to recognize both the recombinant bmk neurotoxin and the one in m. eupeus crude venome. taken together, the molecular characterization and recombinant production of the iranian scorpion m. eupeus venom component can serve as a new probe for further studies ...

background : toxoplasma gondii is an obligate intracellular protozoan parasite, capable of infecting all species of mammals including man. congenital toxoplasmosis is more important during pregnancy for the first time. in this study we expressed and purified p43 toxoplasma gondii tachyzoite and bradyzoite specific surface antigen. methods : the recombinant pgemex-1 contained toxoplasma p43 codi...

conclusions these results demonstrated that the recombinant lysostaphin produced in this study was able to kill nasal s. aureus in rats. it can be recommended for human clinical trial studies. results recombinant lysostaphin at 100 μg/ml concentration showed a significant decrease of the optical density compared to the control samples. the in vivo study demonstrated that a single dose (200 μg/d...