نتایج جستجو برای: real time rt pcr

تعداد نتایج: 2283567  

2008
Izabela Cierlik Lisbeth Jonsson Barbara Karpinska

Plant material and aphid species 8 RNA extraction and measurements of RNA concentration with Nano-Drop 9 RT-PCR and agarose gel electrophoresis 10 Real-Time PCR 11 Bioinformatics: searching for sequences, sequence alignment 13 Results 15 Expression of callose synthase 15 Callose synthase expression analysis with RT-PCR 17 Callose synthase expression analysis with Real-Time PCR 20 Expression of ...

A. K. Goel P. K. Rout S. D. Kharche S. K. Jindal S. Kumar S. Saraswat,

The present study was undertaken to analyze the expression pattern of estrogen receptor 1 gene (ESR1) in Barbari bucks (fertile and non-fertile) identified on the basis of seminal quality traits and fertility trials. RNA was extracted from the spleen by Trizol method. The expression pattern of ESR1 gene was analyzed using real time polymerase chain reaction (RT-PCR). The expression pattern of E...

Journal: :BioTechniques 2005
Lynne V Abruzzo Kathleen Y Lee Alexandra Fuller Alan Silverman Michael J Keating L Jeffrey Medeiros Kevin R Coombes

Profiling studies using microarrays to measure messenger RNA (mRNA) expression frequently identify long lists of differentially expressed genes. Differential expression is often validated using real-time reverse transcription PCR (RT-PCR) assays. In conventional real-time RT-PCR assays, expression is normalized to a control, or housekeeping gene. However, no single housekeeping gene can be used...

شفیعی جندقی, نازنین زهرا, مختاری آزاد, طلعت, کربلایی‌نیا, محمدهادی, یاوریان, ژیلا,

Background: Currently, with increasing risk of influenza A virus epidemics, a lot of studies have been performed. Oseltamivir or Tamiflu (the neuraminidase (NA) inhibitor) is one of the effective drugs for preventing and treatment of these viruses. The H274Y mutation is from the most important drug resistant factors in influenza A viruses. The aim of this study was detection of Oseltamivir...

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