OBJECTIVE Previous studies have reported that the beta and alpha adrenoceptor blocker carvedilol has unique protective effects on free radical-induced myocardial injury. The aim of this study was to examine how carvedilol regulates reactive-oxygen-species-mediated signaling and decreases red blood cell membrane damage in heart perfusion and in a rheological model. METHODS The ischemia-reperfu...

A bacterial strain GSM3, capable of decolorizing an azo dye Reactive Red 2 was isolated from dye contaminated soil sample collected from Ammu dyeing industry, Bellary, India. Based on phenotypic characteristics and 16S rDNA sequencing, strain GSM3 was identified as Pseudomonas aeruginosa GSM3. Isolate GSM3 showed 97% decolorization of Reactive Red 2 (300 mgL) within 10 h, while maximally it cou...

Warm antibody autoimmune hemolytic anemia is due to the presence of warm agglutinins that react with protein antigens on the surface of red blood cells causing premature destruction of circulating red blood cells. We report the first case of concurrent reactive arthritis, Graves' disease, and autoimmune hemolytic anemia. A 40-year-old man with reactive arthritis, Graves' disease, type 2 diabete...

BACKGROUND: The monocyte monolayer assay (MMA) measures the adherence and phagocytosis of sensitized red cells with peripheral monocytes. The result of MMA may reflect the in vivo destruction of sensitized red blood cells (RBCs). STUDY DESIGN AND METHODS: Blood samples from 20 patients with warm reactive autoantibodies were investigated using the MMA. The Monocyte Index (MI) values > 5% were co...

Using an immunocytochemical approach, we have demonstrated a preferential distribution of myosin isoenzymes with respect to the pattern of fiber types in skeletal muscles of the rat. In an earlier study, we had shown that fluorescein-labeled antibody against "white" myosin from the chicken pectoralis stained all the white, intermediate and about half the red fibers of the rat diaphragm, a fast-...

The purpose of this investigation was to test whether the generation of oxygen radicals is required for the cytotoxic activity of human monocytes. The cytotoxic activity of human monocytes was measured as the capacity to lyse, in vitro, human red cells sensitized with noncomplement-binding lgG anti-D alloantibodies. Lysis was determined by measuring 51Cr release from the red cells. Studies were...

The routine pretransfusion red blood cell antibody detection test (PADT), performed at the authors' institution, consists of a threephase, saline-antiglobulin technique (immediate spin, 30-minute incubation at 37 °C, IgG indirect antihuman globulin test |IAT|), and each phase is examined for hemolysis and agglutination. To determine if it would be safe to omit reading the 37 °C phase of the PAD...

This study aims to investigate the anaerobic degradation kinetics of reactive dye, C.I. Reactive Red 141 (Evercion Red H-E7B) by partially granulated anaerobic mixed culture using three carbon sources, namely modified starch (MS), polyvinyl alcohol (PVA) and acrylic size (AS) during batch incubation. There is a first-order kinetics reaction in the decolorization processes using MS and PVA as ca...

In this paper, the effect of NaCl and Na2SO4 on the biodecolourization of reactive brilliant red K-2BP by a Halomonas sp. GYW (EF188281) was investigated in details. The decolourisation efficiency and the oxidation-reduction potential (ORP) change were explored during the decolourization process. The results from sequencing batch tests showed that Na2SO4 influenced the decolourization efficienc...

A novel reactive fluorescent hydrogen sulphide (H2S) probe (T-DNBS) consisting of 2,4-dinitrobenzenesulfonyl (DNBS) units has been synthesised from 4,7-dibromo-2,1,3-benzothidiazole. Adding H2S to T-DNBS solution resulted in a marked fluorescence increase accompanied by visual colour change colourless pale red. Probe performed excellent sensitivity and selectivity for H2S, substantial enhanceme...