BACKGROUND Current methods to determine gene dosage are time-consuming and labor-intensive. We describe a new and rapid method to assess gene copy number for identification of DNA duplications or deletions occurring in Charcot-Marie-Tooth disease type 1A (CMT1A) and hereditary neuropathy with liability to pressure palsies (HNPP), respectively. METHODS We studied 16 patients with HNPP, 4 with ...

The study was carried out to evaluate two rapid diagnostic rabies diagnostic tests in South Eastern Nigeria. One hundred samples each of saliva and brains were collected before and after slaughter from apparently healthy dogs brought for slaughter. The saliva was subjected to rapid immune chromatographic test while the brain tissues were subjected Direct Fluorescent Antibody Test (DFAT) and dir...

The COVID-19 pandemic has underscored the need for rapid high-throughput methods sensitive and specific serological detection of infection with novel pathogens, such as SARS-CoV-2. Multiplex testing can be particularly useful because it simultaneously analyze antibodies to multiple antigens that optimizes pathogen coverage, controls variability in organism individual host response. Here we desc...

A simple hemagglutination inhibition (HI) test for the serological diagnosis of toxoplasmosis has been developed and evaluated. A total of 84 human and 120 mouse serum samples were tested by the newly developed HI test and compared with an immunoglobulin G-indirect fluorescent antibody test. Statistical analysis of serum titers obtained by using the HI test and the immunoglobulin G-indirect flu...

background: recently, the anti cancer effect of chromene derivatives in different cell types has been reported. in this study, we report some derivatives of dihydropyrano[c]chromenes with growth inhibitory and apoptotic activity against k562, chronic myeloid leukaemia (cml). methods: the cells were seeded in 96-well plates at 1×105 cells/well and treated with different concentrations of the dih...