Abstract Aims: The present study aims to investigate the association between catecholamine metabolites (normetanephrine [NMN] and metanephrine [MN]) polycystic ovarian syndrome (PCOS) its clinical manifestations. Materials methods: A total of 60 female patients (18-37 years) were included in study. Twenty-five healthy women 35 with PCOS provided blood urine samples. Anthropometric, biochemical,...

This study aimed to prepare the basic components of aflatoxin B1 (AfB1) radioimmunoassay (RIA) technique using liquid phase to measureAfB1 in agricultural commodities. These components were polyclonal antibody (anti-AfB1), I-labelledAfB1 and aflatoxin B1 standards.Anti-AfB1 polyclonal was raised by immunizing six white New-Zealand rabbits with aflatoxin B1bovine serum albumin (AfB1-BSA) conjuga...

A radioimmunoassay procedure for thyroxine (14) is described, for which only 25 il of serum is required. The antibody, commercially available, is produced in rabbits in response to injections of a T4-albumin conjugate. Native T4-binding proteins are denatured by heating 25 .tl of serum in 0.2 ml of a glutamate buffer in a boiling water bath. After a 1-h incubation of heat-treated serum with l12...

A simple direct radioimmunoassay for 11-deoxycortisol in serum is described. A new type of bridge heterologous 125I labeled 11-deoxycortisol was prepared and was applied to the direct radioimmunoassay. Low and high levels of 11-deoxycortisol in serum were sensitively determined by the direct radioimmunoassay.

Alternative methods to the utilization of laboratory animal blood and its by-products are particularly attractive, especially regarding hamsters due to their small size and difficulties in obtaining serial blood samples. Steroid hormone metabolite quantification in feces, widely used in studies of free-ranging or intractable animals, is a non-invasive, non-stressor, economical, and animal savin...

A radioimmunoassay involving an 125I ligand has been developed and applied to the measurement of urinary metanephrine. To validate the clinical usefulness of this assay, we compared measurement of metanephrine by radioimmunoassay and of total urinary metanephrines by the Pisano colorimetric method. The radioimmunoassay is specific for metanephrine, whereas the colorimetric method measures both ...

The Agency's promotional role in the application of radioimmunoassay to human health is simply summarized: to help developing countries establish effective radioimmunoassay services, and to encourage the application of radioimmunoassay techniques to those health problems in which they can make the greatest contribution. The Agency's expertise is related more to techniques than to medical proble...

A solid-phase radioimmunoassay (Ausria-125) was compared to an immunoelectro-osmophoresis method in detecting hepatitis-B antigen (HBAg). The radioimmunoassay detected HBAg in approximately 230 times higher dilutions than the immunoelectroosmophoresis method. In cases of acute hepatitis HBAg could be detected for about 70% longer after the onset of jaundice using radioimmunoassay compared with ...

Pergolide, a synthetic ergoline with potent dopaminergic activity, is used to treat Parkinson disease. The low plasma concentrations of pergolide achieved during therapy complicate the development of a method for its analysis. Because radioimmunoassay successfully measures other structurally related ergolines in physiological fluids, we undertook the development of a radioimmunoassay of pergoli...