The Molecular Inversion Probe (MIP) assay has been previously applied to a large-scale human SNP detection. Here we describe the PathogenMip Assay, a complete protocol for probe production and applied approaches to pathogen detection. We have demonstrated the utility of this assay with an initial set of 24 probes targeting the most clinically relevant HPV genotypes associated with cervical canc...

background while hepatitis e virus (hev) mostly causes self-limited disease in general population, it is more severe in pregnant women. objectives this study aimed to investigate the seroprevalence of anti-hev igg among a population of pregnant women in west azerbaijan of . patients and methods one hundred thirty six pregnant women referred to urban health centers of urmia for pursuing pregnanc...

introduction: the native inability of nervous system to regenerate, encourage researchers to consider neural tissue engineering as a potential treatment for spinal cord injuries. considering the suitable characteristics of induced pluripotent stem cells (ipscs) for tissue regeneration applications, in this study we investigated the adhesion, viability and proliferation of neural progenitors (de...

background: it is established that sperm dna integrity is essential in fertilization and normal embryo and fetal development. routine semen analysis gives an approximate evaluation of the functional competence of spermatozoa, but does not always reflect the quality of sperm dna. therefore, the evaluation of sperm dna integrity, in addition to routine sperm parameters, could add further informat...

materials and methods a total of 228 fecal samples were collected from residents of rural areas of hamadan, west of iran. each fecal sample was divided into two parts, one kept frozen at -20˚c for ag-capture elisa and the other in 10% formalin for the ap staining method. cryptosporidium ag-detection elisa procedure was performed according to the manual of the manufacturer. the preserved samples...

conclusions antioxidant activity of this essential oil could be somehow attributed to high contents of carvone, carvacrol and thymol. the volatile oil of this plant could have the potential of other biological activities, so further experimental investigations are recommended on this essential oil. results analyses of yellow colored essential oil yielded 88.22% of total oil with 67 components. ...

background: the accuracy of diagnostic assays in human papillomavirus (hpv) genital infection and cervical cancer has remained a clinical challenge in diagnosis.  evidence indicates that a large proportion of cervical cancer can be prevented through organized care for hpv and testing. countries with low per capita income, such as iran and its neighbours, have no national organized program for c...

nowadays, growing interest in the application of nanotechnology in biomedical and biotechnological fields has opened a new avenue to explore the nanoparticles-biological system interaction. indeed, a clear gap is still in the cytotoxic effect of nps on the biological systems. for this purpose, the interaction of the silica oxide nanoparticles (sio2-np) with pc12 cell line, as a model of nervous...