Seven strains of Shope fibroma virus were compared for their effect on rabbit cells in vitro. All but one of the naturally occurring strains examined in this study produced a similar response in the infected cultures. This consisted of continued cell multiplication together with changes in cell morphology and growth pattern. In contrast, a recently isolated strain of fibroma virus, the M1 strai...

In Australia, the rabbit haemorrhagic disease virus (RHDV) has been used since 1996 to reduce numbers of introduced European rabbits (Oryctolagus cuniculus) which have a devastating impact on the native Australian environment. RHDV causes regular, short disease outbreaks, but little is known about how the virus persists and survives between epidemics. We examined the initial spread of RHDV to s...

An enzyme-linked immunoabsorbent assay was developed for detection and quantification of the yolk protein lipovitellin (Lv) and its plasma precursor, vitellogenin (Vg), in winter flounder (Pleuronectes americanus). Native Lv was found to be a mixture of heat-stable and heat-labile molecules in mature, ovulated eggs. A heat-stable Lv fraction was purified from extracts of unfertilized eggs by br...

Intracytoplasmic sperm injection is the newest and the most successful micromanipulation technique for treating male factor infertility. It entails the mechanical insertion of a chosen spermatozoon directly in to the cytoplasm of an oocyte. The technique was used in veterinary science for the purpose of clarifying the different steps of fertilization by investigating the fusion and penetration ...

RH-0345 (halofenozide), a bisacylhydrazine derivative, is a nonsteroidal ecdysteroid agonist that mimics the action of the moulting hormones, while KK-42, an imidazole compound, is a potent inhibitor of ecdysteroid biosynthesis. Previous results suggested that the observed reduction of ecdysteroid titre, leading to a reduction of reproductive capacity in the mealworm Tenebrio molitor, is due to...

In this study we examined the efficiency of an in vitro feeding technique using glass microcapillaries as a method of establishing rickettsiae-infected lines of ticks. To quantify the volume ingested by ticks during microcapillary feeding, the incorporation of radiolabeled amino acids in tick gut and hemolymph was calculated. Fifteen of 18 ticks consumed between 0.06 and 6.77 microl. However, i...

Myxovirus parainfluenza-3 (SF-4 bovine strain) was first isolated in tissue cultures of embryonic bovine kidney (EBK) cells (Reisinger, Heddleston, and Manthei, J. Am. Vet. Med. Assoc. 135:147, 1959). This agent is antigenically and biologically related to the human HA-1 strain isolated previously by Chanock et al. in monkey kidney cells (Abinanti and Huebner, Virology 8:391, 1959; Abinanti et ...

In this study, we produced a polyclonal antibody against unprocessed chicken myostatin and examined the effect of in ovo administration of the antibody on posthatch chicken growth and muscle mass. A PCR-amplified unprocessed chicken myostatin cDNA was cloned into an Escherichia coli expression vector, and myostatin proteins were expressed. Recombinant myostatin purified by electro-elution of th...

Female rabbits and mice were actively immunized against germ cell antigen (GA-1) of 63 kDa molecular mass isolated from rabbit sperm and testis. There was a significant (P less than 0.05) reduction of fertility in rabbits actively immunized with GA-1 as compared to controls, as seen by the percentage of 9-day implants/corpora lutea ratio (GA-1, 36.3%; controls, 85.7%). In mice, there was again ...

background: the effect of temperature and humidity on the maturation of toxocara cati eggs in an in vitro system was investigated. methods: suspensions of toxocara cati eggs, with 5% formalin/saline or 2.5% formalin/ringer were prepared and maintained at 37 °c under 40% humidity or at 25 °c under 98% humidity for 3 weeks for egg development. results: the suspension sample mixed by 2.5% formalin...