نتایج جستجو برای: quantitative rt pcr

تعداد نتایج: 481030  

Journal: :Annals of Microbiology 2023

Abstract Purpose Coral degradation is a worldwide ecological problem. Bacterial diseases are great danger to coral health. The pathogenic bacterium Vibrio alginolyticus XSBZ14 isolated from diseased had been identified as the of Porites andrewsi White syndrome (PAWS) in Xisha Archipelago on transmission experiment. To date, molecular mechanism by which this pathogen causes disease unknown, and ...

Journal: :Molecular human reproduction 2009
R G Craythorn J E Girling M P Hedger P A W Rogers W R Winnall

Identifying suitable housekeeping genes for quantitative RT-PCR in the uterus is problematic, as this tissue undergoes significant structural and functional alterations during the oestrous cycle and pregnancy in response to circulating hormones. The suitability of 18S rRNA as a housekeeping gene in mouse uterus was investigated by introducing an 'RNA spike' standard into the reverse transcripti...

Journal: :Methods 2001
M S Rajeevan D G Ranamukhaarachchi S D Vernon E R Unger

Real-time reverse transcription polymerase chain reaction (RT-PCR) methods that monitor product accumulation were adapted for the validation of differentially expressed genes. We describe a real-time quantitative PCR assay that uses SYBR Green I dye-based detection and product melting curve analysis to validate differentially expressed genes identified by gene expression profiling technologies....

Journal: :American journal of clinical pathology 2008
Massimo Barberis Caterina Pellegrini Maria Cannone Carmelo Arizzi Guido Coggi Silvano Bosari

We performed a technical and cost-effectiveness analysis of quantitative reverse transcription-polymerase chain reaction (Q-RT-PCR) for the assessment of HER2 in breast cancer. We evaluated 44 frozen and 55 formalin-fixed paraffin-embedded (FFPE) breast carcinoma specimens by Q-RT-PCR, immunohistochemical analysis, and fluorescent in situ hybridization (FISH). Immunohistochemical and FISH analy...

Journal: :BioTechniques 1996
C J Elferink J J Reiners

A quantitative reverse transcription polymerase chain reaction (RT-PCR) assay was developed to amplify a region of the CYP1A1 heterogeneous nuclear RNA (hnRNA) transcript encompassing the first intron-exon boundary. The RT-PCR protocol uses a CYP1A1 recombinant RNA internal standard identical to the target hnRNA except for an engineered unique internal restriction site. Its inclusion enables no...

Journal: :BioTechniques 1996

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