GOGOL P. 2013. Motility parameters and intracellular ATP content of rabbit spermatozoa stored for 3 days at 15°C. Folia Biologica (Kraków) 61: 87-91. The effect of semen storage duration on motility parameters and ATP content of rabbit spermatozoa were investigated. Ejaculates were collected from 9 New Zealand White male rabbits and diluted with a commercial rabbit semen extender Galap. Semen w...

The present work studied different spermatozoa parameters and the ability of frozen rabbit spermatozoa to fertilize, in vitro, in vivo-matured oocytes, as a test to predict their in vivo fertility and prolificacy. Semen from rabbit bucks was frozen using two freezing protocols [in a freezer at -30 degrees C or in liquid nitrogen vapour (LNV)]. For the in vivo trial, females were inseminated wit...

Reactive oxygen species (ROS) have a role in the modulation of gamete quality and gamete interaction. Generation of ROS is inherent in spermatozoa and contaminating leukocytes. ROS influence spermatozoa, oocytes, embryos and their environment. Oxidative stress (OS) mediates peroxidative damage to the sperm membrane and induces nuclear DNA damage. ROS can modulate the fertilizing capabilities of...

Cholesterol, being the starting point of steroid hormone synthesis, is a long known modulator of both female and male reproductive physiology especially at the level of the gonads and the impact cholesterol has on gametogenesis. Less is known about the effects cholesterol homeostasis may have on postgonadic reproductive functions. Lately, several data have been reported showing how imbalanced c...

in human fertilization, the sperm centrosome nucleates a radial array of microtubules called the sperm aster. the sperm aster is responsible for apposition of male and female pronuclei, and later gives rise to the first meiotic spindle. the objective of this study was to determine microtubule assembly and chromatin configuration in rabbit oocytes following intracytoplasmic injection with human ...

Background: Polycystic ovary syndrome (PCOS) is a multifactorial, heterogeneous, complex genetic endocrine and metabolic disorder, diagnostically characterized by chronic anovulation, polycystic ovaries and biochemical and clinical manifestations of hyperandrogenism. In patients with PCOS lower apoptotic rates found in granulosa cells compared to women with regular ovulatory cycles and this lea...

Fertilization marks the end of oogenesis and the beginning of embryogenesis. The legacy of oogenesis is, however, maintained by virtue of the stored nutritional and regulatory molecules that fuel, and in many cases direct, early development. The signal that stimulates the transition from oocyte to embryo is a sperm-induced increase in the concentration of intracellular free Ca2+. Thus successfu...