N-acyl and acetoxy derivatives of putrescine and cadaverine have been found in the faeces of children and in cultures of isolates of gut bacteria. The evidence was accumulated from two dimensional, thin layer chromatography, field desorption mass spectrometry, and accurate mass measurement of the DANS derivatives of the amines. The acetoxy compounds of putrescine and cadaverine have not previou...

Drought stress is a significant environmental variable affecting wheat growth and development. Plant tolerance intimately related to regulators of plants as polyamines. The study assessed the impact drought (50% water irrigation 100% irrigation), priming grains in putrescine (0.25, 0.5, 1 mM), their interactions on growth, yield, physiological attributes plants. conditions declined plant height...

Hypoxic pulmonary vascular remodeling in rats is associated with increased polyamine transport in pulmonary artery smooth muscle cells (PASMCs). We therefore defined constitutive and hypoxia-induced polyamine transport properties of rat cultured PASMCs and determined the impact of polyamine transport blockade on hypoxia-induced accumulation of p38 MAP kinase. PASMCs exhibited polyamine transpor...

Nondigestible but fermentable dietary fructans such as oligofructose exert many effects on gut physiology through their fermentation end products such as short-chain fatty acids. Could other metabolites be produced in the gut and contribute to the physiologic effects of dietary fructans? The aim of the study was to evaluate the influence of oligofructose on putrescine, spermidine and spermine c...

Polyamine uptake, the kinetics of this uptake, and the competition among polyamines and between polyamines and inorganic cations were studied in petals of Saintpaulia ionantha Wendl. Uptake experiments using (14)C-labeled polyamines were carried out on single petals, at room temperaure (20 degrees C) and in the light. The results show that putrescine, spermidine, and spermine uptake was depende...

The migration of IEC-6 cells is inhibited when the cells are depleted of polyamines by inhibiting ornithine decarboxylase with alpha-difluoromethylornithine (DFMO). Exogenous putrescine, spermidine, and spermine completely restore cell migration inhibited by DFMO. Because polyamines are interconverted during their synthesis and catabolism, the specific role of individual polyamines in intestina...

A series of diamine homologues of putrescine and triamine homologues of spermidine was used to determine the structural specificity of the polyamine transport system in ascites L1210 leukemia cells by measuring their ability to compete with [3H]-putrescine, [3H]spermidine, or [3H]spermine for uptake. Transport specificity among the diamines (as indicated by K1 constants) was greatest for those ...

Ornithine decarboxylase (EC 4.1.1.17), the enzyme that catalyzes the synthesis of putrescine from ornithine, increases dramatically in developing Xenopus embryos. Between the 2-cell stage and early blastula stage, activity increases 10-fold, and in swimming tadpoles, the enzyme activity is 100-fold higher than that present in either unfertilized eggs or 2-cell embryos. S-adenosyl-L-methionine d...

This study was conducted during two successive seasons 2005 and 2006 by using "Desert Red" peach cultivar (Prunus persica L.) grafted on Nemaguard rootstock. Trees were sprayed with a hand sprayer to the run off at the initiation of phase ΙΙΙ of the double sigmoid curve before the onset of the climacteric. The treatments included putrescine at 5 and 10 mM, GA3 at 200 and 400 ppm, in addition to...