expression of htlv-i p19 protein in an escherichia coli expression system always leads to the formation of inclusion body. solubilisation and refolding of the inclusion bodies is complex, time consuming and difficult during large-scale preparation. this study aimed to express and purify a soluble form of recombinant htlv-i p19 protein in an e. coli expression system. the synthetic dna encoding ...

the purification of biomolecules is a necessary step in many biochemical researches. in this regard, developments of convenient, specific and low cost methods of purification are of particular interest. given the human hemoglobin (hb) affinity toward some charged carbohydrates, interaction of this molecule with human chorionic gonadotropin (hcg) which is a glycoprotein hormone containing sialic...

our previous study revealed that the antioxidant activity of polysaccharide (coded as fgfp) extracted from grifola frondosa by enzymolysis treatment was significantly superior than that (coded as gfp) extracted by boiling-water. in this study, one purified polysaccharide fractions (coded as fgfp-11) was obtained from fgfp by purified using deae-52 column and sephacryl s-500hr column. results in...

abstract during a survey for detection of pvs in potato fields in northern khorasan province, some tubers from the plants which have been already marked and showing mosaic, rugosity, undulation on leaf margins, little leaf and slightly vein deepening, were collected in bodjunrd region, also, some tubers from potato fields of mashhad, chenaran, ghoochan, faruj and shirvan regions were randomly c...

polyphenol oxidase (ppo) from tomatoes was extracted and partially purified through  (nh4)2so4 precipitation, dialysis and ion exchange chromatography. the activity of polyphenol oxidase was investigated in solanum lycopersicum. spectrophotometric method was used to assay the enzyme activity and the kinetic constants - maximum enzyme velocity (vmax) and michealis - menten constant (km). of the ...

background and objectives: brucella melitensis infection is still a major health problem for human and cattle in developing countries and the middle east. materials and methods: in this study, in order to screen immunogenic candidate antigens for the development of a brucella subunit vaccine, a cytoplasmic protein (dnak) and an outer membrane protein (omp31) of b. melitensis were cloned, expres...

polyphenol oxidase (ec. 1.10.3.1 ppo), an ionically unbound and thermostable enzyme, was extracted from the leaves of cinnamomum tamala having aroma flavor used to add in indian food and vegetables as a part of spices to enhance the taste of recipes. the enzyme was purified 2.63-fold with a total yield of 9.5% by ammonium sulfate precipitation followed by sephadex g-100 gel filtration chromatog...

bacillus sp. guf8, isolated from acidic soil samples of a tea farm was identified as bacillus cereus, based on 16s rdna sequencing and standard bacterial identification methods. following optimization of enzyme production, the resulting α-amylase was purified by acetone precipitation and ion exchange chromatography. consequently, thermostability and kinetic parameters of the purified enzyme wer...

in this study, we present changes of peroxidase (e. c. 1.11.1. 7) activities in the root of brassica napus var. okapi during rosette stage until ripening of the fruits. peroxidase activity was determined seasonally over an eight month period. results showed that total peroxidase activity in the root was highest in mid june and the lowest activities were observed from time of winter dormancy unt...

milk clotting enzyme (m ce) produced by: rhizomucor miehei was purified and characterized.the enzyme was purified 220.29-fold with specific activity about 14444.2 u/mg protein byultrafiltration, ammonium sulfate fractionation, sephacryl s-300 chromatography. the maximumenzyme activity was at 65°c.the milk clotting activity was decreased steadily as ph is increased and indicated maximumactivity ...