Pseudomonas sp. strain ADP is the model strain for studying bacterial degradation of the s-triazine herbicide atrazine. In this work, we focused on the expression of the atzDEF operon, involved in mineralization of the central intermediate of the pathway, cyanuric acid. Expression analysis of atzD-lacZ fusions in Pseudomonas sp. strain ADP and Pseudomonas putida showed that atzDEF is subjected ...

A bacterial strain (designated IH5(T)), isolated from rhizospheric soil of grasses growing spontaneously in Spanish soil, actively solubilized phosphates in vitro when bicalcium phosphate was used as a phosphorus source. This strain was Gram-negative, strictly aerobic, rod-shaped and motile. The strain produced catalase, but not oxidase. Cellulose, casein, starch, gelatin, aesculin and urea wer...

A mutant of Pseudomonas sp. strain HBP1, originally isolated on 2-hydroxybiphenyl, was selected for the ability to grow on 2-propylphenol as the sole carbon and energy source. In the mutant strain, which was designated as Pseudomonas sp. strain HBP1 Prp, the cellular induction mechanism involved in the synthesis of the NADH-dependent monooxygenase is changed. 2-Propylphenol, which is known to b...

Pseudomonas sp. strain T and Pseudomonas sp. strain K172 grow with toluene under denitrifying conditions. We demonstrated that anaerobic degradation of toluene was initiated by direct oxidation of the methyl group. Benzaldehyde and benzoate accumulated sequentially after toluene was added when cell suspensions were incubated at 5 degrees C. Strain T also grows anaerobically with m-xylene, and w...

Pseudomonas is a complex genus with increasing numbers of new species. Recently, we isolated sp. strain L22-9, which showed antifungal activity against several fungal phytopathogens. Here, report the whole genome sequence L22-9. Genomic analysis revealed that L22-9 contains one circular DNA chromosome 6,730,360 bp length 60.9% GC content. Bioinformatics identified gene clusters in synthesize an...

Treatment of solid and liquid tobacco wastes with high nicotine content remains a longstanding challenge. Here, we explored an environmentally friendly approach to replace tobacco waste disposal with resource recovery by genetically engineering Pseudomonas putida. The biosynthesis of 3-succinoyl-pyridine (SP), a precursor in the production of hypotensive agents, from the tobacco waste was devel...

Sulfur-limited batch enrichment cultures containing one of nine multisubstituted naphthalenesulfonates and an inoculum from sewage yielded several taxa of bacteria which could quantitatively utilize 19 sulfonated aromatic compounds as the sole sulfur source for growth. Growth yields were about 4 kg of protein per mol of sulfur. Specific degradation rates were about 4 to 14 mu kat/kg of protein....

Primers were selected from Shapir et al. 2008 with the one base pair correction listed in the Material and Methods section. Primer specificity was tested by nucleotide BLAST search analysis (NCBI, http://blast.ncbi.nlm.nih.gov/Blast.cgi) for possible binding partners in Pseudomonas sp. strain ADP chromosomal and plasmid DNA, as well as for binding sites on identified sequences obtained from exo...

An entomopathogenic, Gram-negative bacterium isolated from a female specimen of the fruit fly Drosophila melanogaster was taxonomically characterised. Strain L48(T) was strictly aerobic, non-fermentative, oxidase and catalase positive, rod-shaped, and motile due to a polar inserted flagellum. Phylogenetic analysis of the 16S rRNA gene and three other housekeeping genes placed strain L48 (T) in ...