Background: Wound infection is a highly common problem in hospital settings, where microbes are often resistant and difficult to treat due rapid exposure antibiotics. While treating wound infection, bacteria enter the deep tissue; as therapy needs long time, have sufficient time develop biofilm, which makes them much more Objectives: The current study was performed identify wound-infecting dete...

Recently, the search for novel products derived from natural sources has become necessary due to decreasing effectiveness of current antibiotics in treating bacteria that are antibiotic-resistant. In this context, it is well known entomopathogenic (EPBs) produce a broad range secondary metabolites with antibacterial activities. Therefore, an in-vitro trial was conducted isolate and identify non...

Two novel 3-chlorobenzoate-degrading bacteria were previously isolated from an aquifer in which no such bacteria could be enriched prior to the introduction of the 3-chlorobenzoate-degrading strain, Pseudomonas sp. B13. To understand the origin of 3-chlorobenzoate-degrading genes in the two novel isolates, the 16S ribosomal RNA, clcD (dienelactone hydrolase) and clcA (chlorocatechol oxygenase) ...

Four nitrite-dissimilating strains, isolated from Weser Estuary sediments, were investigated using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains belong to the 'Betaproteobacteria' and are related to the genus Alcaligenes. The highest level of sequence similarity (100 %) was found with strain M3A (=ATCC 700596), a dimethyl su...

To analyze the status of the genus Alcaligenes in the agricultural environment, we developed a PCR method for detection of these species from vegetables and farming soil. The selected PCR primers amplified a 107-bp fragment of the 16S rRNA gene in a specific PCR assay with a detection limit of 1.06 pg of pure culture DNA, corresponding to DNA extracted from approximately 23 cells of Alcaligenes...

Comparative 16S rRNA gene sequence analysis indicates that two distinct sublineages exist within the genus Alcaligenes: the Alcaligenes faecalis lineage, comprising Alcaligenes aquatilis and A. faecalis (with the three subspecies A. faecalis subsp. faecalis, A. faecalis subsp. parafaecalis and A. faecalis subsp. phenolicus), and the Alcaligenes defragrans lineage, comprising A. defragrans. This...

background: infections are readily transmitted as a result of bacterial contamination of swimming pools. therefore, hygiene and preventing the contamination of swimming pools is of particular importance. the objective of this study was to determine the amount of bacterial contamination in indoor pools of yazd in 2013. methods: in this descriptive and analytical study, all indoor swimming pools ...