The extent of glycation and conformational changes of horse myoglobin (Mb) upon glycation with N-acetyl-glucosamine (GlcNAc), glucose (Glc) and glucosamine (GlcN) were investigated. Among tested sugars, the rate of glycation with GlcN was the most rapid as shown by MALDI and ESI mass spectrometries. Protein oxidation, as evaluated by the amount of carbonyl groups present on Mb, was found to inc...

Non-enzymatic protein glycation is a source of metabolic stress that contributes to cytotoxicity and tissue damage. Hyperglycemia has been linked to elevation of advanced glycation endproducts, which mediate much of the vascular pathology leading to diabetic complications. Enhanced glycation of immunoglobulins and their accelerated vascular clearance is proposed as a natural mechanism to interc...

Plantagoside (5,7,4',5'-tetrahydroxyflavanone-3'-O-glucoside) and its aglycone (5,7,3',4',5'-pentahydroxyflavanone), isolated from a 50% ethanol extract of Plantago major seeds (Plantaginaceae), were established to be potent inhibitors of the Maillard reaction. These compounds also inhibited the formation of advanced glycation end products in proteins in physiological conditions and inhibited p...

Carboxylated osteocalcin (Gla-OC) contributes to the bone formation, whereas its undercarboxylated form (Glu-OC) takes part in the energy metabolism. In vitro studies had shown that treatment of osteoblast-like cells with advanced glycation end product-modified bovine serum resulted in reduced synthesis of collagen 1 and osteocalcin. The aim of this study was to find association between Gla-OC ...

BACKGROUND Glucose-induced protein glycation has been implicated in the progression of diabetic complications and age-related diseases. The anti-glycation potential of polyphenol-rich plant extracts has been shown previously. Bunium Persicum has been demonstrated to possess a high level of polyphenols. The aim of current in vitro study was to determine the possible inhibitory effect of Bunium P...

human serum albumin has been used as a model protein for protein folding and ligand binding studies over many decades. due to its long life period and high concentration in plasma, hsa is highly sensitive to glycation. it is reported that 175 mg/dl glucose concentration is a threshold of kidney activity for the beginning of excretion of glucose. ph denaturation of hsa in absence and presence of...

Diabetes mellitus is a group of metabolic diseases that, due to the long-term pathological effect hyperglycaemia on tissues, lead development typical chronic organ complications. Chronic leads increased activity polyol pathway with subsequent accumulation sorbitol and fructose, formation end products advanced glycation, alteration protein kinase C activity, excessive reactive oxygen species ass...

Diabetes mellitus is a chronic metabolic disorder, and diabetic complications lead to morbidity and death in diabetic patients. Hyperglycemia has been shown to be the most important risk factor in charge for the systemic complications. Various biochemical pathways have been proposed to explain the adverse effects of hyperglycemia. Potential cellular mechanisms of hyperglycemia-induced complicat...

Glycation refers to the non-enzymatic molecular interaction between carbonyl group of sugars and amino groups macromolecules viz. proteins, DNA, lipids leading generation Schiff’s base, Amadori products, finally converted deleterious advanced glycation end-products (AGEs). Several diseases such as neurodegenerative or mental disorders, cardiovascular complications, well diabetes, its related co...

OBJECTIVE To assess the relative importance of fasting and postprandial hyperglycemia to vascular dysfunction in diabetes, we have measured indicators of glycation, oxidative and nitrosative stress in subjects with type 1 diabetes, and different postprandial glucose patterns. RESEARCH DESIGN AND METHODS Plasma and urinary levels of specific arginine- and lysine-derived advanced glycation end ...