isolation and purification of major outer membrane proteins (omp) from the cell wall envelope of brucella abortus s-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (tx-100) and lysozyme treatments, followed by ultracentrifugation. the crude omp was treated with trypsin to free the preparation from any other protein contaminants. t...

helicobacter pylori (h.pylori) is an etiologic factor for chronic gastritis and peptic ulcers. serological testing of h.pylori infection is common in iran, as other parts of the world. there are geographical variations in the humoral immune response to various h. pylori strains in different parts of the worl. we studied the immunogenic proteins of h.pylori by means of an immunoblot assay with a...

background: the effects of extremely low frequency electromagnetic fields (elf-emf) on toxoplasma gondii have not been explained yet. the aim of this study was to assess the possible effects of elf-emf on growth, survival time and viability of toxoplasma gondii . in addition, the life span of toxoplasma infected animals was investigated. methods: sixty adult male balb/c mice were used for in vi...

 Background: Candida species are among the most common causes of opportunistic fungal diseases. Among Candida species, Candida albicans is responsible for most infections. Having many strains, C. albicans is very polymorph. C. dubliniensis is very similar to albicans species both morphologically and physiologically. For an infection to occur, cell wall proteins play an important role as they en...

Larrea et al. recently reported that patients with multiple myeloma (MM) who achieve complete remission (CR) after induction chemotherapy with novel agents (glucocorticoids with bortezomib, lenalidomide, thalidomide, or bortezomib with thalidomide) are more likely to have the emergence of oligoclonal bands in peripheral blood. While only 11% of patients who received conventional chemotherapy de...

Topical prednisolone and cortexolone, a known glucocorticoid receptor antagonist, differentially affected the synthesis of proteins in normal corneal epithelium and migrating conjunctival epithelium after complete corneal deepithelialization, as measured by 35S-methionine incorporation and SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel) electrophoresis. Application of either prednisolone o...

We have previously shown that autologous dendritic cells (DCs) pulsed with tumour cell lysate and cultured with autologous T cells from patients with B-cell chronic lymphocytic leukaemia (B-CLL) stimulate significant increases in proliferation, IFN-gamma secretion and specific HLA class II-restricted cytotoxicity to B-CLL targets. In this study, normal and tumour cell lysates were analysed by r...

In the study of tissues that contain several forms of one cytochrome P450 subfamily, it is useful to develop immunoblotting techniques so that the various individual members of the family can be distinguished. This paper describes improvements in the immunoblotting technique to distinguish members of the rat cytochrome P450 4A subfamily, 4A1, 4A2, and 4A3, as they are present in Sprague-Dawley ...

objective: pyocyanine plays an important role in the pathogenesis of pseudomonas aeruginosa, (p. aeruginosa) and is known to have inhibitory and bactericidal effects. this study has aimed to detect the phenazine biosynthetic operon (phz abcdefg) and two phenazine modifying genes (phzm and phzs) by polymerase chain reaction (pcr) and detection of its possible protein bands by sodium dodecyl sulf...