Objective: Investigate plant natural products with inhibitory activity of Leishmania proteases, because the inhibition certain proteases induce parasite death. Methods: Descriptive/exploratory study, integrative review type. The search was performed in following databases: Scientific Electronic Library OnLine (SciELO), Medical Literature Analysis and Retrieval System Online (MEDLINE), Latin Ame...

The pandemic caused by the new coronavirus has resulted in a global health emergency and prompted an urgent need for treatment strategies. No target-specific drugs are currently available SARS-CoV-2, but drug candidates targeting viral replication cycle being explored. A prime target of drug-discovery efforts is SARS-CoV-2 main protease (Mpro). In this work, we identified potential inhibitor us...

The objective of this study was focussed on phytochemical screening, in vitro antioxidant and antiprotease activities various solvent extracts Muehlenbeckia platyclada root. roots were washed thoroughly, shade dried coarsely powdered. powdered material successively extracted with hexane, chloroform methanol using soxhlet apparatus. Preliminary screening for carbohydrates, proteins, alkaloids, p...

BACKGROUND The HIV protease is essential for the life cycle of the virus and is an important target for the development of therapeutic treatments against AIDS. The structures of HIV protease in complex with different inhibitors have helped in understanding the interactions between inhibitors and the protease and in the design and optimization of HIV protease inhibitors. RESULTS We report here...

Human kallikrein (hK) 2 is an arginine-selective serine protease expressed predominantly in the prostate that has an 80% sequence identity with prostate-specific antigen. Expression of hK2 is elevated in the tumor epithelium compared to benign prostate tissue. We have purified, sequenced, and identified a novel hK2 complex in prostate tissue consisting of hK2 and a serine protease inhibitor kno...

Protease inhibitors can be versatile tools mainly in the fields of medicine, agriculture and food preservative applications. Fungi have been recognized as sources of protease inhibitors, although there are only few such reports on mushrooms. This work reports the purification and characterization of a trypsin inhibitor from the fruiting body of edible mushroom Pleurotus floridanus (PfTI) and it...

This case report describes two severe antiretroviral drug adverse reactions that occurred in the same patient. A 55-year-old HIV-positive African woman received a single epidural triamcinolone injection for pain relief of postherpetic neuralgia. Forty-one days later, she developed severe iatrogenic Cushing's syndrome due to the drug-drug interaction between triamcinolone and her boosted proteas...

This paper reports the presence of a Kunitztype protease inhibitor (HGPI) gene in Dolichos biflorus for the first time. A full-length protease inhibitor gene with complete open reading frame of 669 bp encoding 222 amino acids was cloned from D. biflorus using a PCRbased method. BlastN search showed that the HGPI gene shared 100% homology with Cicer arietinum trypsin inhibitor mRNA and 97% with ...

All members of the human herpesvirus protease (HHV Pr) family are active as weakly associating dimers but inactive as monomers. A small-molecule allosteric inhibitor of Kaposi's sarcoma-associated herpesvirus protease (KSHV Pr) traps the enzyme in an inactive monomeric state where the C-terminal helices are unfolded and the hydrophobic dimer interface is exposed. NMR titration studies demonstra...

A 2.1-kb cDNA clone representing a new member of the protease inhibitor family was isolated from a human liver cDNA library. The inhibitor, named human Leuserpin 2 (hLS2), comprises 480 ami no acids and contains a leucine residue at its putative reactive center. HLS2 is about 2 5-28% homologous to three huaan members of the plasma protease inhibitor family: antithrombin III, o^l-antitrypsin and...