Although several methods for RNA isolation from plants have been reported (3,4,7,10), there are no methods described for RNA isolation from plant cell suspension cultures and calli. During the characterization of transgene expression in tobacco cell suspension cultures and calli, we observed that it is difficult and tedious to isolate RNA from limited amounts of those cells frozen in liquid nit...

This research was carried out to investigate the effects of chemical mutagens on producing phenolic compounds in calli obtained from Royal grape variety. Petioles were cultured B5 medium (0.5 mg/L benzyl amino purine and 0.5 indole acetic acid) obtain callus. Calli subcultured same nutrient three times at one month intervals. In fourth subculture, transferred media with composition containing e...

Freezing stress affects the geographic distribution, growth, and development of potato, resulting in yield loss. Solanum malmeanum, a diploid wild species with strong freezing tolerance, was fused sensitive dihaploid S. tuberosum by somatic hybridization. In our study, 980 calli were obtained, 248 differentiated shoots obtained from calli. Parental-specific SSR markers used to analyse chromosom...

Dehydrin is known to have an important role in plant response and adaptation abiotic stresses including drought high salinity. Previous research reported the isolation of full-length coding sequence (CDS) DHN1 from sugarcane var. PSJT 941, it shares a homology with DHN genes sorghum other varieties. In this study, CDS was cloned under constitutive CaMV35S promoter transformed into calli mediate...