The mechanism of action of two forms of the prolactin (PRL) receptor was studied using analogs of human growth hormone (hGH). At low concentrations (approximately 1 pM), hGH binds and stimulates proliferation of Nb2 cells containing the 391-residue PRL receptor as well as murine lymphoid FDC-P1 cells transfected with the 591-residue hPRL receptor. However, at high concentrations (approximately ...

Investigations over two decades have revised understanding of the prolactin hormone. Long thought to be merely a lactogenic hormone, its list of functions has been extended to include: reproduction, islet differentiation, adipocyte control and immune modulation. Prolactin functions by binding cell-surface expressed prolactin receptor, initiating signaling cascades, primarily utilizing Janus kin...

Members of the cytokine/growth hormone/prolactin (PRL) receptor superfamily are associated with cytoplasmic tyrosine kinases of the Jak family. For the PRL receptor (PRLR), after PRL stimulation, both the kinase Jak2 and the receptor undergo tyrosine phosphorylation. To assess the role of tyrosine phosphorylation of the PRLR in signal transduction, several mutant forms of the PRLR in which vari...

a total of 125 russian red pied cows were genotyped for the prolactim-related gene. the prl-rsai genotypes were analysed using the polymerase chain reaction -restriction fragment length polymorphism (pcr-rflp) method. in this breed, the frequencies of alleles were as follows;  a= 0.794 and b= 0.206. the frequencies of aa, ab and bb genotypes were 0.598, 0.392 and 0.01; respectively. results sho...

The 16 kDa N-terminal fragment of prolactin (16K-prolactin) is a potent antiangiogenic factor. Here, we demonstrate that matrix metalloproteases (MMPs) produced and secreted by chondrocytes generate biologically functional 16K-prolactin from full-length prolactin. When incubated with human prolactin at neutral pH, chondrocyte extracts and conditioned medium, as well as chondrocytes in culture, ...

A cDNA encoding a putative non-membrane bound prolactin receptor was amplified by RT-PCR from red deer (Cervus elaphus) testis. Sequence analysis suggests that the testicular cDNA is generated by alternative splicing resulting in the deletion of exons 7 and 8, which code for: (a) the final 53 aa of the extracellular domain of the receptor including the fifth conserved cysteine residue and the W...

The transcription factor nuclear factor1-C2 (NF1-C2) mediates the action of prolactin in the mammary gland. Research on the molecular genetic mechanism of model system have indicated that the NF1-C2 gene plays an important role for the activation of several mammary gland specific genes (Nilsson et al. 2006). Moreover, the effects of prolactin on milk production traits have been reported in rumi...

The hormone combination of insulin, dexamethasone and prolactin induced accumulation of preproepidermal growth factor (EGF) mRNA in HC11 mouse mammary epithelial cells 16-24 h after the hormones were added to the cultures. Individual hormones or combinations of two of the hormones had no effect on EGF mRNA concentrations. The same hormone combination was capable of inducing expression of a repo...

Prophet of POU1F1 (PROP1) plays direct or indirect important roles in the morphogenesis of pituitary gonadotropes as well as lactotropes, somatotropes and caudomedial thyrotropes. It also controls the expression of growth hormone, prolactin and thyrotropin subunits through regulatory PIT1 factor. These hormones have important influences on production traits in most animal species including the ...

introduction: macroprolactin is a significant cause of misdiagnosis, unnecessary investigation, and inappropriate treatment in patients with hyperprolactinemia. its frequency has not been clearly established due to technical difficulties in identifying it. most laboratories and clinicians are unaware of macroprolactin interferences in prolactin assays. materials and methods: a comprehensive lit...