background: spermatozoa preservation is an approach to improve the fertility rate to pass on the valuable genetic material from sire to their offspring. during the last 20 years, reproductive biotechnologists have focused on the approaches that improve spermatozoa cryosurvival. one of the possible mechanisms is supplementation in semen extender. objectives: the aim of this study was to evaluate...

Background: Adipose tissue is not only an energy storage tissue but also an important endocrine organ that secretes protein hormones. Among them, the production and secretion of adiponectin is inversely correlated to the severity of obesity. In adipose tissue, adiponectin production is transcriptionally regulated by peroxisome proliferator-activated receptor-γ (PPAR-γ). Obesity is a major healt...

The aim of this study was to report a successful pregnancy using completely immotile frozen-thawed spermatozoa selected by laser. A single laser shot was used to detect the presence of viable immotile spermatozoa in fresh and frozen-thawed testicular spermatozoa. The viability rate was 55.8% after the laser detection, and cryopreservation was carried out immediately. The thawing test was perfor...

In this study, quantitive characteristics and chemical composition of in roach (Rutilus rutilus caspicus) sperm were investigated. Sperm traits included sperm movement duration, percentage of motile spermatozoa, sperm density, spermatocrit and sperm volume. Some seminal plasma characteristics (sodium, potassium, calcium, magnesium and chloride) were investigated. In addition, some metabolites o...

The purpose of this study was to characterize highly enriched human spermatozoa membrane proteins by two-dimensional electrophoresis and computer image analysis. Sperm membrane proteins were extracted by detergent solubilization from three different preparations: 1) washed semen cells following centrifugation and three wash steps in Ham's F-10 medium (the standard sperm preparation, which is co...

Experiments were conducted to determine the efficiency with which viable, morphologically normal bovine spermatozoa could be isolated using a discontinuous bovine serum albumin (BSA) gradient. In the first experiment, extended semen was layered on top of a BSA gradient (4% BSA over 10% BSA) contained in a 500-ml separatory funnel. When comparing 1, 3, 5, 7, 14 or 21 X 10(9) spermatozoa applied ...

GOGOL P. 2013. Motility parameters and intracellular ATP content of rabbit spermatozoa stored for 3 days at 15°C. Folia Biologica (Kraków) 61: 87-91. The effect of semen storage duration on motility parameters and ATP content of rabbit spermatozoa were investigated. Ejaculates were collected from 9 New Zealand White male rabbits and diluted with a commercial rabbit semen extender Galap. Semen w...

The objective of this study was to use a microfluidic sperm sorter (MFSS), designed to isolate motile human spermatozoa with laminar flows (no centrifugation), for porcine IVF. Boar spermatozoa were diluted at 1 x 10(8) cells/mL with a diluent containing 20% seminal fluid and flowed with modified TCM-199 (mM199, with 5 mM caffeine) to introduce motile sperm into the exit chamber for IVF. In Exp...

The present study was aimed to determine the effect of GnRH analogue [des-Gly10, D-Ala6]-LH-RH ethylamide on the quality of rabbit spermatozoa stored at 17°C for 3 days. Semen from 5 bucks (13 ejaculates) was used in the experiment. Ejaculates were divided and diluted at a 1:10 ratio with rabbit semen extender Galap (IMV, France) (Control) or with Galap extender supplemented with GnRH analogue ...