Preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis has been used to purify digitonin extracts of rod outer segments of squid retinas. Besides rhodopsin the extracts contained small amounts of seven or eight contaminating proteins plus a light-stable pink-purple pigment identified as ommin. Reproducible amino acid analyses were obtained from separate purified preparations of o...

In the present paper (1) a method of preparative zone electrophoresis (cf. 2, 3) is described in which a stiff starch gel with only 7 to 8 per cent of solid constituents is used as a supporting medium. Two simple devices will be described, one for the fractionation of 2 ml. of protein solution and the other for processing up to 50 ml. of solution, containing 5 to 10 per cent of protein mixture....

Histidine decarboxylase was purified 800-fold from the kidneys of thyroxine-treated mice. The purification procedure included precipitation of protein from a crude supernatant after heating it to 55 degrees C at pH 5.5, fractionation with (NH4)2SO4, phosphocellulose column chromatography, chromatofocusing, DEAE-Sepharose column chromatography, gel filtration on Sephacryl S-300 and preparative p...

Among the antimicrobial phytoalexins produced by Phaseolus vulgaris (French bean) are the prenylated isoflavonoids kievitone and phaseollidin. Two enzyme activities, kievitone hydratase and phaseollidin hydratase, occur in culture filtrates of the bean pathogen, Fusarium solani f. sp. phaseoli, and catalyse similar hydration reactions on the dimethylallyl moieties of the phytoalexins. The enzym...

We isolated and purified to homogeneity a caseinolytic protease from a Nocardia brasiliensis cell extract. Preparative polyacrylamide gel electrophoresis and electroelution were employed for purification. This purified protease was injected in BALB/c mice and induced IgM and IgG anti-protease antibodies. Active immunization of mice with this protease prevented mycetoma development in experiment...

Organic constituents of human saliva vary around 0.5 g. per 100 ml. depending on the nature of the stimulus and the hour of collection. Most of the organic matter is protein, with mucin being the preponderant component. However, it is one of the smaller protein elements that has been the most widely studied, mainly because of its enzymatic action, that is, salivary amylase. Phase boundary elect...

Snake venom is a complex mixture of proteins, peptides, enzymes, carbohydrates, and minerals. They contain a variety of chemicals with pharmacological and toxicological properties. The innate immune system is the first line of defense against toxins and microbes. Antibacterial and anticancer proteins produced by snake venom have recently attracted significant attention due to their relevance to...

A method to obtain C1r, a subunit of the first complement component, in a highly purified state has been described for the first time. The stepwise method starts with a neutral euglobulin precipitation, after diethylaminoethyl- and carboxymethyl-cellulose chromatography and a final preparative polyacrylamide electrophoresis step. Such C1r preparations are devoid of C1q and C1s activities and sh...

A naturally occurring inhibitor of activated blood coagulation Factor X has been isolated from pooled rabbit plasma and purified by the combination of Sephadex G-ZOO gel filtration, DEAE-Sephadex A-SO, and DEAE-cellulose chromatography. On microzone electrophoresis the purified product traveled as an az-globulin, and on 7.5% polyacrylamide gel disc electrophoresis at pH 9.5 it traveled as a sin...