BACKGROUND Despite interest in cryopreservation of individual or small number of human spermatozoa, to date, little data is available as regards its effectiveness. We systematically reviewed the outcome after cryopreservation of individual or small numbers of human spermatozoa in patients with severe male factor of infertility. METHODS We searched the MEDLINE, EMBASE, Cochrane Systematic Revi...

This study examined the effect of metal ions Ca2+, Mg2+, and Mn2+ and sonication on ejaculated frozen-thawed bovine sperm adenylate cyclase activity, and whether cAMP levels in sperm changed during capacitation with heparin. The sperm adenylate cyclase was almost insensitive to Ca2+ at concentrations up to 10 mM when cold-shocked homogenized spermatozoa were used. Adenylate cyclase activity, as...

Adult stem cells show excellent perspectives for applications in the field of regenerative medicine. In the last two decades, great strides have been made in spermatogonial stem cell research showing the potentiality of these stem cells in organ regeneration. Spermatogonial stem cells, derived from post-natal or adult mice, not only differentiate into cell types other than the spermatozoa when ...

OBJECTIVE To study the resistance of human spermatozoa to cryoinjury in repeated cycles of thaw-refreezing by using the fast liquid nitrogen vapor method. MATERIALS AND METHODS Semen specimens were obtained from sixteen normal and oligozoospermic individuals who required disposal at the sperm bank. Five of them had testicular cancer. Specimens were thawed and an aliquot was removed for analys...

background: leptin and its receptor are present in spermatozoa; however, the role of leptin in sperm function is still controversial. our present study aimed at demonstrating the effect of cryopreservation on sperm dna fragmentation (dnaf) and investigating the possible effects of sperm capacitation techniques and leptin in vitro incubation on frozen-thawed sperm dnaf and oxidative stress. meth...

The present work studied different spermatozoa parameters and the ability of frozen rabbit spermatozoa to fertilize, in vitro, in vivo-matured oocytes, as a test to predict their in vivo fertility and prolificacy. Semen from rabbit bucks was frozen using two freezing protocols [in a freezer at -30 degrees C or in liquid nitrogen vapour (LNV)]. For the in vivo trial, females were inseminated wit...

in the present study, the fertilizing potential of semen recovered from slaughtered bulls epididymis was evaluated after cryopreservation, by conventional techniques and flow cytometry methods. the cauda epididymal was dissected and sperm were recovered and evaluated for volume, sperm concentration, and membrane and acrosome integrity using a flow cytometer. sperm fertility potential was tested...

Conventional sperm freezing methods perform best when freezing sperm samples containing at least hundreds of spermatozoa. In this severe male factor infertility case series, we examined the reproductive outcomes in 12 intracytoplasmic sperm injection cases where spermatozoa used were frozen in Cell Sleepers. Cell Sleepers are novel devices in which individual spermatozoa can be frozen in microd...

Objective(s):Althoughroutinely applied in assisted reproductive technology, human sperm cryopreservation is not a completely successful procedure. Adverse effects of cryopreservation on the fertilization capacity, motility, morphology, and viability of spermatozoa have been proven; cryopreservation has also shown a role in sperm DNA fragmentation and infertility. The post-thaw survival of sperm...

Transgenesis constitutes an important tool for pharmacological protein production and livestock improvement. We evaluated the potential of laparoscopic insemination (LI), in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) to produce egfp-expressing ovine embryos, using spermatozoa previously exposed to pCX-EGFP plasmid in two different sperm/DNA incubation treatments: "Lon...