Gene replacement in yeast is often accomplished by using a counterselectable marker such as URA3. Although ura3 strains of Pichia pastoris have been generated, these strains are inconvenient to work with because they grow slowly, even in the presence of uracil. To overcome this limitation, we have developed an alternative counterselectable marker that can be used in any P. pastoris strain. This...

background: helicobacter pylori strains have two classical virulence genes, the cytotoxinassociated a ( caga ) gene and the vacuolating cytotoxin a ( vaca ) gene, which are located in the cag pathogenicity island ( cag pai). serum immunoglobulin g (igg) antibodies to h. pylori , especially, the caga antigen may be a reliable marker for selection of dyspeptic patients for upper endoscopy. method...

Plant genetic engineering has, until now, relied on the incorporation of foreign DNA into plant genomes. Public concern about the extent to which transgenic crops differ from their traditionally bred counterparts has resulted in molecular strategies and gene choices that limit, but not eliminate, the introduction of foreign DNA. Here, we demonstrate that a plant-derived (P-) DNA fragment can be...

despite the importance of drought stress in early stage of growth and plant establishment, it has usually been neglected as selection criteria in breeding programs. therefore, a population of recombinant inbred line derived from the cross “babax/seri m82” and their two parents were studied. the experiment was conducted in two randomized complete block designs with two replications under osmotic...

Aliphatic glucosinolates are the predominant sulfur-rich plant secondary metabolites in economically important Brassica crops. Glucosinolates and their hydrolysis products are involved in plant-microbe, plant-insect, plant-animal, and plant-human interactions. It is, therefore, important to manipulate glucosinolate profiles and contents in Brassica species. In this study, aliphatic glucosinolat...