The purpose of the present study was to evaluate the usefulness of rpoB-PCR assay and concentration flurochrome (CF) staining against culture for direct detection of mycobacteria sp in respiratory specimen amongsymptomatic patients. Of the 900 specimens 52 were culture positive with Löwenstein-Jensen Medium (LJM). Of the 52 culture positive samples, 48 were positive by rpoB-PCR (sensitivity 92....

Septic arthritis in children can be difficult to diagnose and may be associated with severe morbidity. A majority of apparent septic arthritis cases may have negative culture, thereby creating a dilemma regarding treatment. The medical charts of 209 children with the diagnosis of septic arthritis were retrospectively reviewed to evaluate the differences between culture-negative (n = 64) and cul...

  Background : An accurate and prompt diagnosis of bacterial arthritis is essential for earlier treatment and a good outcome. Superantigens produced by Staph. Aureus are among the most lethal toxins. The paper objective was Identification of common bacterial antigens and S.aureus superantigens in synovial fluid (SF) of children with negative culture and direct smear for other bacteria except fo...

introduction: ureaplasma uriealyticum is one of the most important causes of nongonococcal and nonspecific urethritis (ngu & nsu) in men. mycoplasma hominis too has a causal role in ngu & nsu. this study aimed to investigate whether it is possible to detect mycoplasma hominis and ureaplasma uriealyticum in first voided urine samples in men suffering from ngu & nsu without complaints of urethral...

Microbiologic cultures are not infrequently negative in patients with a histopathologic diagnosis of chronic osteomyelitis. Culture-negative cases may represent low-grade infections with a lower metabolic activity than culture-positive cases. 18F-FDG PET could potentially detect such a difference. We determined whether the level of 18F-FDG PET uptake differs in patients with culture-negative an...

Conventional culture methods to detect methicillin-resistant Staphylococcus aureus (MRSA) take a few days, and their sensitivity and usefulness also need to be improved. In this study, active screening was performed using the polymerase chain reaction (PCR) for colonization with MRSA on admission and follow-up surveillance after admission to an emergency department between June 2012 and August ...

AIMS The purpose of this study was to determine the clinical significance of detecting microbial footprints of ureaplasmas in amniotic fluid (AF) using specific primers for the polymerase chain reaction (PCR) in patients presenting with cervical insufficiency. METHODS Amniocentesis was performed in 58 patients with acute cervical insufficiency (cervical dilatation, > or =1.5 cm) and intact me...