Abstract Cross-drainage hydraulic structures such as culverts and bridges in urban landscapes are prone to get blocked by the transported debris (e.g., urban, vegetated), which often reduces their capacity triggers flash floods. Unavailability of relevant data from blockage-originated flooding events complex nature accumulation highlighted factors hindering research within blockage management d...

Reducing the gap between electrodes and diaphragm to zero is an often adopted strategy reduce ohmic drop in alkaline water electrolyzers for hydrogen production. We provide a thorough account of current–voltage relationship such zero-gap configuration over wide range electrolyte concentrations current densities. Included are voltage components that not experimentally quantified like those due b...

An understanding of how the nuclear pore complex (NPC) mediates nucleocytoplasmic exchange requires a comprehensive inventory of the molecular components of the NPC and a knowledge of how each component contributes to the overall structure of this large molecular translocation machine. Therefore, we have taken a comprehensive approach to classify all components of the yeast NPC (nucleoporins). ...

The study of the nuclear pore complex (NPC) is a fascinating endeavor, as it not only implies uncovering the 'engineering marvel' of its architecture and function, but also provides a key window into a significant evolutionary event: the origin of the eukaryotic cell. The combined efforts of many groups in the field, with the help of novel methodologies and new model organisms, are facilitating...

In vivo studies on the dynamics of the nuclear pore complex (NPC) in yeast suggested that NPCs are highly mobile in the nuclear envelope. However, new evidence indicates that in mammalian cells NPCs are stably attached to a flexible lamina framework, but a peripheral component can exchange rapidly with an intranuclear pool.

Nuclear pore complexes (NPCs) have been isolated from the yeast Saccharomyces. Negative stain electron microscopy of the isolated NPCs and subsequent image reconstruction revealed the octagonal symmetry and many of the ultrastructural features characteristic of vertebrate NPCs. The overall dimensions of the yeast NPC, both in its isolated form as well as in situ, are smaller than its vertebrate...

To follow the dynamics of nuclear pore distribution in living yeast cells, we have generated fusion proteins between the green fluorescent protein (GFP) and the yeast nucleoporins Nup49p and Nup133p. In nup133- dividing cells that display a constitutive nuclear pore clustering, in vivo analysis of GFP-Nup49p localization revealed changes in the distribution of nuclear pore complex (NPC) cluster...

We employ a two-dimensional spectral element method to investigate the effect of various semi-circular blockages on the laminar flow between two flat plates. This simplified geometry has a general relevance to blocked flows, but more academically, also relates to work on the backward-facing step, presenting a qualitatively similar flow with a single, independent step, or blockage, parameter. Th...

A differential pressure transmitter with an orifice is widely used as a flowmeter. A major drawback of this type of flowmeter is blockage of the impulse line, which connects the differential pressure transmitter to the orifice tap. A diagnostic method based on the amplitude of pressure fluctuations in the impulse line can be used to diagnose a blockage in the steady state; however, there is the...

The process of freezing of water and blockage of pipes are summarized. Current research on freezing and its applications are briefly reviewed. Design concepts are introduced for doing work by utilizing pressures caused by ice blockage. Several conceptual designs are presented for experimental setups for the isostatic pressurization of thin walled tubes. Potential practical uses of pressures gen...