Hemagglutinating antibody responses of Lewis-Wistar and Sprague-Dawley rats to graded doses of type-specific pneumococcal polysaccharide were measured. Rats given a small dose (0.2 to 50 mug)of type 1 or 8 polysaccharide intraperitoneally developed type-specific hemagglutinating antibody. Rats given larger doses of polysaccharide (greater than or equal to200 mug) did not develop detectable hema...

The primary attachment to polymer surfaces followed by accumulation in multilayered cell clusters leads to biofilm production of Staphylococcus epidermidis, which is thought to contribute to virulence in biomaterial-related infections. We purified a specific polysaccharide antigen of biofilm-producing S. epidermidis 1457 and RP62A, which was recently shown to have a function in the accumulative...

Candida albicans is the most prevalent cause of hospital-acquired fungal infections and forms biofilms on indwelling medical devices that are notoriously difficult to treat or remove. We recently demonstrated that the colonization of C. albicans on the surfaces of catheter tube segments can be reduced in vitro by coating them with polyelectrolyte multilayers (PEMs) that release a potent antifun...

F OR practical purposes we may consider that endotoxins of gram-negative bactena are the cause of endotoxic shock. In general, endotoxins are produced by bactena which form smooth colonies. However, it should be mentioned that small quantities of endotoxin have been found in colonially rough, gram-negative rods and in some gram-positive bacteria. Endotoxins are found in the outer layers of the ...

objective(s):sub-health has been described as a chronic condition of unexplained deteriorated physiological function, which falls between health and illness. in the present study, we evaluated the effects of lycium barbarum polysaccharide (lbp), a polysaccharide fraction purified from lycium barbarum (l. barbarum) on the sub-health mice. materials and methods:the sub-health model mice were buil...

Macromolecular heparin, isolated from rat skin, was found by ultracentrifugation to have a molecular weight of >900,000. After treatment of this material with nitrous acid, a core fraction (Mr = 20,000) was isolated that contained more than 90% of the neutral sugars (xylose and galactose) and amino acids (essentially serine and glycine) of the total reaction products but less than 4% of the ura...

Within the biomaterials proposed for tissue regeneration, synthetic 3D hydrogels that mimic soft tissues possess great potential regenerative medicine but their poor vascularization rate is usually incompatible with long-term cell survival. Fabrication of promote and/or accelerate remains nowadays a challenge. In present work, tubular geometries ranging from 28 to 680 μm in diameter, correspond...

Certain strains of Bradyrhizobium japonicum form a previously unknown polysaccharide in the root nodules of soybean plants (Glycine max (L.) Merr.). The polysaccharide accumulates inside of the symbiosome membrane-the plant-derived membrane enclosing the bacteroids. In older nodules (60 days after planting), the polysaccharide occupies most of the symbiosome volume and symbiosomes become enlarg...

A polysaccharide was isolated from the broth of cultured Streptomyces virginia H03 which was treated by ethanol deposition and savage method to remove the protein, and was purified using Sephadex G-150 column chromatography. The components of the polysaccharide were determined by gas chromatography. The purified polysaccharide was made up of mannose, glucose and galactose, in a 2:1:1 proportion...

The objective of this paper was to extract and purify lily polysaccharide and to study its anti-H22 hepatoma effect in mice. Orthogonal experimental method was used to analyze the factors influencing the extraction and purification of lily polysaccharide, and the anti-tumor effect of lily polysaccharide was studied by acting it on H22-bearing mice. The results showed that the size of influence ...