PREMISE OF THE STUDY Transcriptome-derived simple sequence repeat (SSR) markers were developed in Asparagus cochinchinensis (Asparagaceae). Due to its application in traditional medicine, its wild populations are threatened by over-collection even in protected areas, requiring immediate conservation efforts. METHODS AND RESULTS Based on transcriptome data of A. cochinchinensis, 96 primer pair...

BACKGROUND Microsatellites or simple sequence repeats (SSRs) are highly informative molecular markers for various biological studies in plants. In spruce (Picea) and other conifers, the development of single-copy polymorphic genomic microsatellite markers is quite difficult, owing primarily to the large genome size and predominance of repetitive DNA sequences throughout the genome. We have deve...

Simple sequence repeat (SSR) markers were used to authenticate ramets of 11 Persian walnut ( Juglans regia L.) varieties. All varieties and 28 their (n = 39) genotyped with 17 SSR markers. The genetic profiles revealed two off-types: the Serr 4 (S4) Vina 1 (V1). fingerprints individuating possible using 13 polymorphic SSRs that could be in future identify clones these Except for ‘Chandler’, eac...

Microsatellite primers are a valuable tool to use for both observational and experimental studies in numerous taxa. Here, we develop 18 16 microsatellite markers the widespread duckweeds Lemna minor Spirodela polyrhiza, respectively. Only four were not polymorphic when tested on samples from Europe Western Pennsylvania, USA.

the association between 140 microsatellite bands of 20 primer pairs and 13 quantitative traits recorded on 70 aegilops crassa from iran was evaluated. pearson’s coefficients showed significant correlations between some morphological traits. polymorphic information contents (pic) of loci were in the range of 0/16 (xgwm190) to 0/44 (xgwm161). the result of multiple regression analysis showed sign...

Finger millet is an important cereal crop in eastern Africa and southern India with excellent grain storage quality and unique ability to thrive in extreme environmental conditions. Since negligible attention has been paid to improving this crop to date, the current study used Next Generation Sequencing (NGS) technologies to develop both Simple Sequence Repeat (SSR) and Single Nucleotide Polymo...

Nuclear sequence markers are useful tool for the study of the history of populations and adaptation. However, it is not easy to obtain multiple nuclear primers for organisms with poor or no genomic sequence information. Here we used the genomes of organisms that have been fully sequenced to design comprehensive sets of primers to amplify polymorphic genomic fragments of multiple nuclear genes i...

Carvalho M., Matos M., Carnide V., 2014. Fingerprinting of Vaccinium corymbosum cultivars using DNA of fruits. Hort. Sci. (Prague), 41: 175–184. In recent years the production and consumption Vaccinium corymbosum has increased. Highbush blueberry cultivars are divided into three types, northern, intermediate and southern. The traditional methods for classification of highbush blueberry cultivar...

ziziphora tenuior, named kakooti in farsi, is one of the aromatic and medicinal plants of a wide distribution in iran. in this study, rapd molecular markers, (by utilization of 16 primers) was applied to determine the genetic diversity of 39 samples of ziziphora tenuior from 21 geographical regions in iran. a number of 100 polymorphic bands out of 110 amplified ones was produced and analyzed th...

Black rot caused by Xanthomonas campestris pv. campestris (Pam.) Dowson is the most destructive disease of cauliflower causing huge loss to the farmers throughout the world. Since there are limited sources of resistance to black rot in B. oleracea (C genome Brassica), exploration of A and B genomes of Brassica was planned as these were thought to be potential reservoirs of black rot resistance ...