Neural Wiskott-Aldrich syndrome protein (N-WASP) regulates reorganization of the actin cytoskeleton through activation of the Arp2/3 complex. Here, we show that heat shock protein 90 (HSP90) regulates N-WASP-induced actin polymerization in cooperation with phosphorylation of N-WASP. HSP90 binds directly to N-WASP, but binding alone does not affect the rate of N-WASP/Arp2/3 complex-induced in vi...

Graft polyrotaxanes, with poly(ε-caprolactone) (PCL) graft chains on the ring components were synthesized by the simultaneous ring-opening polymerization of ε-caprolactone from both ends of the backbone polymer, an end-functionalized polyethylene glycol (PEG) and the formation of inclusion complexes with α-cyclodextrin (α-CD). PEG with multiple functional groups at each end was prepared by the ...

Emulsion polymerization reactions constitute complex, nonequilibrium systems in which there is strong interaction between polymers, monomers, and the colloidal structures that mediate the reactions. A novel and general method was sought to monitor emulsion polymerization reactions. Instrumentation and methods were hence developed here to achieve, for the first time, simultaneous monitoring of t...

A growing body of data supports a view of the actin cytoskeleton of smooth muscle cells as a dynamic structure that plays an integral role in regulating the development of mechanical tension and the material properties of smooth muscle tissues. The increase in the proportion of filamentous actin that occurs in response to the stimulation of smooth muscle cells and the essential role of stimulus...

ethylene was polymerized in slurry phase over a ticl4/mg(oc2h5)2 catalyst activated by al(c2h5)3. it was shown experimentally that mass transfer resistance has no effect on polymerization kinetics. after estimation ofthe active site concentration by inhibition method with carbon monoxide, the effects of polymerization temperature, monomer and cocatalyst  oncentrations and hydrogen were investig...

Background and Aim: Minimizing the polymerization shrinkage of composite resins is a critical goals in composite restorations. Many factors affect polymerization shrinkage. This study aimed to evaluate the effect of composite resin shade on polymerization shrinkage. Materials and Methods: In this in vitro study, 14 composite discs measuring 1.5×8 mm were fabricated. The samples were prepared u...

T-cell antigen receptor (TCR) engagement induces formation of multi-protein signalling complexes essential for regulating T-cell functions. Generation of a complex of SLP-76, Nck and VAV1 is crucial for regulation of the actin machinery. We define the composition, stoichiometry and specificity of interactions in the SLP-76, Nck and VAV1 complex. Our data reveal that this complex can contain one...

The Arp2/3 complex is implicated in actin polymerization-driven movement of Listeria monocytogenes. Here, we find that Arp2p and Arc15p, two subunits of this complex, show tight, actin-independent association with isolated yeast mitochondria. Arp2p colocalizes with mitochondria. Consistent with this result, we detect Arp2p-dependent formation of actin clouds around mitochondria in intact yeast....

Aqueous coordination polymerization of ethylene by different neutral nickel(II) complexes [(X∧O)NiR(L)] (X ) P: type 1; X ) N: type 2) was investigated. With catalyst precursors of type 1, productivities and molecular weights were reduced by comparison to conventional polymerization in organic solvents. This effect results to a large extent from a lowering of the chain growth rate due to a low ...