نتایج جستجو برای: polymerase chain reactionn

تعداد نتایج: 352457  

ژورنال: :مجله دانشکده پزشکی اصفهان 0
عبدالمجید فتی استاد، گروه انگل شناسی و قارچ شناسی، دانشکده ی پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران سید عبدالرحیم رضایی استاد، گروه ایمونولوژی، دانشکده ی پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران الهام مقدس استادیار، گروه انگل شناسی و قارچ شناسی، دانشکده ی پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران فائزه سادات موسوی وفا دانشجوی کارشناسی ارشد، گروه انگل شناسی و قارچ شناسی، دانشکده ی پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران سید علی اکبر شمسیان دانشیار، گروه انگل شناسی و قارچ شناسی، دانشکده ی پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران

مقدمه: leishmaniasis پوستی، از بیماری های انگلی پوستی است که در بسیاری مناطق جهان به ویژه ایران به صورت اندمیک وجود دارد. هر چند روش میکروسکوپی روش متداول تشخیص بیماری می باشد، اما تشخیص گونه های عامل leishmaniasis به صورت میکروسکوپی و یا از طریق یافته های بالینی امکان پذیر نیست. برای این منظور، روش های مولکولی از اهمیت ویژه ای برخوردار هستند. روش ها: در این مطالعه، شناسایی گونه ی عامل leishman...

Journal: :iranian journal of microbiology 0
yashgin hassanzadeh department of microbiology, science and research branch, islamic azad university, fars, iran. nima bahador department of microbiology, science and research branch, islamic azad university, fars, iran. majid baseri-salehi department of microbiology, kazeroun branch, islamic azad university, kazeroun, iran.

background and objective: photobacterium damselae subsp. damselae is a marine pathogenic bacterium which causes disease in marine animals and human. this bacterium mostly found in coastal shallow seawater. so, the aim of this study was isolation and characterization of photobacterium damselae subsp. damselae from edible fish of persian gulf, bandar abbas. material and methods: totally 100 fish ...

Journal: :jundishapur journal of microbiology 0
nader divan khosroshahi cellular and molecular research centre, qazvin university of medical sciences, qazvin, ir iran taghi naserpour farivar cellular and molecular research centre, qazvin university of medical sciences, qazvin, ir iran; cellular and molecular research centre, qazvin university of medical sciences, qazvin, ir iran. tel: +98-9128801401, fax: +98-2833324971. pouran johari cellular and molecular research centre, qazvin university of medical sciences, qazvin, ir iran

background legionellaceae contains legionella genus with over 52 species and 64 serogroups. it is one of the most important causes of respiratory disease in human. more than 30% of hospital-acquired pneumonia is caused by legionella. ventilator-associated pneumonia (vap) is an infection acquired in hospital wards, particularly in intensive care unit (icu). this disease approximately affects 9% ...

Journal: :jundishapur journal of microbiology 0
shahrzad soltani department of medical parasitology, ahvaz jundishapur university of medical sciences, ahvaz, ir iran shahram khademvatan department of medical parasitology, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; cellular and molecular research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran jasem saki department of medical parasitology, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; health research institute, infectious and tropical diseases research center, ahvaz jundishapur university of medical science, ahvaz, ir iran; department of medical parasitology, ahvaz jundishapur university of medical sciences, ahvaz, ir iran. po box: 613715794. tel: +98-6113367543-50, fax: +98-6113332036 heshmatollah shahbazian department of nephrology, golestan hospital and diabetes research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran

results 34 (34%) of the renal transplant recipients and 26 (26%) of the control group were positive for anti-toxoplasma igg. in addition 18 (18%) of the renal transplant recipients and 4 (4%) of the control group were positive for anti-toxoplasma igm. for both values of the antibodies, differences were statistically significant (p < 0.025 and p < 0.001 respectively). the pcr results indicated t...

Journal: :jundishapur journal of microbiology 0
ramezan ali ataee department of medical microbiology, faculty of medicine, baqiyatallah university of medical sciences, tehran, ir iran; department of medical microbiology, baqiyatallah university of medical sciences, mollasadra ave, p. o. box: 193955487, tehran, ir iran. tel: +98-9122190418, fax: +98-26127258

Journal: :jundishapur journal of microbiology 0
keivan majidzadeh tasnim biotechnology of research center (tbrc), faculty of medicine, aja university of medical sciences, tehran, ir iran; cancer genetics research group, breast cancer research center (bcrc), acecr, tehran, ir iran amirhossein mohseni tasnim biotechnology of research center (tbrc), faculty of medicine, aja university of medical sciences, tehran, ir iran mohammad soleimani tasnim biotechnology of research center (tbrc), faculty of medicine, aja university of medical sciences, tehran, ir iran; tasnim biotechnology research center (tbrc), aja university of medical sciences, etemadzadeh ave, west fatemi, tehran, ir iran. tel: +98-2188337928, fax: +98-2188337928

conclusions: the results showed that direct fusion of the recombinant plasmids containing the initial and ending parts of the target gene are simple and cost-effective techniques for increasing the length of the fragment and constructing ipc. results: the size of the pcr product was equal to the sum of the first and second fragments. sequencing confirmed the presence of the desire insert (ipc s...

Journal: :jundishapur journal of microbiology 0
elahe mirzaie-kashani department of biology, faculty of science, university of isfahan, isfahan, ir iran majid bouzari department of biology, faculty of science, university of isfahan, isfahan, ir iran; department of biology, faculty of science, university of isfahan, isfahan, ir iran. tel.: +98-3117932459, fax: +98-3117932456 ardeshir talebi pathology laboratory, faculty of medicine, al-zahra university hospital, isfahan university of medical sciences, isfahan, ir iran farahnaz arbabzadeh-zavareh department of operative dentistry, faculty of dentistry, isfahan university of medical sciences, isfahan, ir iran

background cervical cancer is the second most common cancer in women worldwide. recent studies show that human papillomavirus (hpv) dna is present in all cervical carcinomas and in some cervicitis cases, with some geographical variation in viral subtypes. therefore determination of the presence of hpv in the general population of each region can help reveal the role of these viruses in tumors. ...

Journal: :international journal of reproductive biomedicine 0
naeime mohseni moghadam babak kheirkhah toraj reza mirshekari majid fasihi harandi elham tafsiri

background: mycoplasmas can cause acute and chronic diseases at multiple sites with wide-range complications and have been implicated as cofactors in diseases. the infections influenced form genital mycoplasmas specifically mycoplasma hominis and mycoplasma genitalium potentially affect reproductive disorders, and infertility. objective: isolation and molecular identification of mycoplasma geni...

Journal: :jundishapur journal of microbiology 0
elahe beiranvand department of parasitology and mycology, school of medicine, shiraz university of medical sciences, shiraz, ir iran mohsen kalantari mamasani paramedical school, shiraz university of medical sciences, shiraz, ir iran hasan ali rastgar department of medical entomology and vector control, school of public health, student research committee, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; poledokhtar health centre, lorestan university of medical sciences, khorramabad, ir iran kamyar amraee department of medical entomology and vector control, school of public health, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; department of medical entomology and vector control, school of public health, ahvaz jundishapur university of medical sciences, ahvaz, ir iran. tel/fax: +98-6113738282

background cutaneous leishmaniasis with zoonotic and anthroponotic forms is an endemic disease and a health problem in our country. identification of parasite species and the type of disease is very important for treatment of disease as well as planning the control program. this study was carried out to identify the parasite species causing cutaneous leishmaniasis by nested pcr in poledokhtar d...

2009
Kjell Kleppe

INTRODUCTION On April 25, 1953 James D. Watson and Francis Crick publish "a radically different structure" for DNA, thereby founding the field of Molecular Genetics. Their structure involves two strands of complementary base-paired DNA, running in opposite directions as a double helix. They conclude their report saying that "It has not escaped our notice that the specific pairing we have postul...

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