results 34 (34%) of the renal transplant recipients and 26 (26%) of the control group were positive for anti-toxoplasma igg. in addition 18 (18%) of the renal transplant recipients and 4 (4%) of the control group were positive for anti-toxoplasma igm. for both values of the antibodies, differences were statistically significant (p < 0.025 and p < 0.001 respectively). the pcr results indicated t...

conclusions: the results showed that direct fusion of the recombinant plasmids containing the initial and ending parts of the target gene are simple and cost-effective techniques for increasing the length of the fragment and constructing ipc. results: the size of the pcr product was equal to the sum of the first and second fragments. sequencing confirmed the presence of the desire insert (ipc s...

background cervical cancer is the second most common cancer in women worldwide. recent studies show that human papillomavirus (hpv) dna is present in all cervical carcinomas and in some cervicitis cases, with some geographical variation in viral subtypes. therefore determination of the presence of hpv in the general population of each region can help reveal the role of these viruses in tumors. ...

background: mycoplasmas can cause acute and chronic diseases at multiple sites with wide-range complications and have been implicated as cofactors in diseases. the infections influenced form genital mycoplasmas specifically mycoplasma hominis and mycoplasma genitalium potentially affect reproductive disorders, and infertility. objective: isolation and molecular identification of mycoplasma geni...

background cutaneous leishmaniasis with zoonotic and anthroponotic forms is an endemic disease and a health problem in our country. identification of parasite species and the type of disease is very important for treatment of disease as well as planning the control program. this study was carried out to identify the parasite species causing cutaneous leishmaniasis by nested pcr in poledokhtar d...

INTRODUCTION On April 25, 1953 James D. Watson and Francis Crick publish "a radically different structure" for DNA, thereby founding the field of Molecular Genetics. Their structure involves two strands of complementary base-paired DNA, running in opposite directions as a double helix. They conclude their report saying that "It has not escaped our notice that the specific pairing we have postul...

George Maltezos, Alvaro Gomez, Jiang Zhong, Frank A. Gomez, and Axel Scherer Department of Electrical Engineering, California Institute of Technology, 1200 East California Boulevard, MS 200-36 Pasadena, California 91125, USA Department of Chemistry and Biochemistry, California State University, Los Angeles, 5151 State University Drive, Los Angeles, California 90032-8202, USA Keck School of Medi...

s The polymerase chain reaction (PCR) is a technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. PCR is now a common and often indispensable technique used in medical and biological research labs for a variety of applications. There are three major steps in...

This essay on the polymerase chain reaction is one of a series developed as part of FASEB's efforts to educate the general public, and the legislators whom it elects, about the benefits of fundamental biomedical research-particularly how investment in such research leads to scientific progress, improved health, and economic well-being.