نتایج جستجو برای: polyketide synthase gene

تعداد نتایج: 1200120  

2015
Bahgat Fayed David A Ashford Amal M Hashem Magdy A Amin Omaima N El Gazayerly Matthew A Gregory Margaret C M Smith

Bacteria in the genus Streptomyces and its close relatives are prolific producers of secondary metabolites with antibiotic activity. Genome sequencing of these bacteria has revealed a rich source of potentially new antibiotic pathways, whose products have never been observed. Moreover, these new pathways can provide novel genes that could be used in combinatorial biosynthesis approaches to gene...

Journal: :Chemical science 2015
Joleen Masschelein Charlien Clauwers Ufedo R Awodi Karen Stalmans Wesley Vermaelen Eveline Lescrinier Abram Aertsen Chris Michiels Gregory L Challis Rob Lavigne

The zeamines are a unique group of antibiotics produced by Serratia plymuthica RVH1 that contain variable hybrid peptide-polyketide moieties connected to a common pentaamino-hydroxyalkyl chain. They exhibit potent activity against a broad spectrum of Gram-positive and Gram-negative bacteria. Here we report a combination of targeted gene deletions, high resolution LC-MS(/MS) analyses, in vitro b...

Journal: :Journal of bacteriology 1992
B Shen R G Summers H Gramajo M J Bibb C R Hutchinson

The acyl carrier protein (ACP) of the tetracenomycin C polyketide synthase, encoded by the tcmM gene, has been expressed in both Streptomyces glaucescens and Escherichia coli and purified to homogeneity. Expression of the tcmM gene in E. coli results mainly in the TcmM apo-ACP, whereas expression in S. glaucescens yields solely the holo-ACP. The purified holo-TcmM is active in a malonyl coenzym...

2010
Leah C. Blasiak Jon Clardy

Genome mining and identification of natural product gene clusters typically relies on the presence of canonical nonribosomal polypeptide synthetase (NRPS) or polyketide synthase (PKS) domains. Recently, other condensation enzymes, such as the ATP-grasp ligases, have been recognized as important players in natural product biosynthesis. In this study, sequence based searching for homologues of Dd...

Journal: :The Plant cell 2001
N Yalpani D J Altier E Barbour A L Cigan C J Scelonge

Salicylic acid (SA) has been shown to act as a signal molecule that is produced by many plants subsequent to the recognition of potentially pathogenic microbes. Increases in levels of SA often trigger the activation of plant defenses and can result in increased resistance to subsequent challenge by pathogens. We observed that the polyketide 6-methylsalicylic acid (6-MeSA), a compound that appar...

Journal: :Journal of molecular microbiology and biotechnology 2002
Christopher J Wilkinson Zoë A Hughes-Thomas Christine J Martin Ines Böhm Tatiana Mironenko Matthew Deacon Michael Wheatcroft Gabriele Wirtz James Staunton Peter F Leadlay

An Escherichia coli-actinomycete shuttle vector, pCJW93, was constructed which places cloned genes under the control of the thiostrepton-inducible tip promoter from Streptomyces lividans. We also constructed expression vectors bearing the actII-ORF4/PactI activator-promoter system of the actinorhodin biosynthetic pathway of Streptomyces coelicolor. With both types of vector, levels of expressio...

Journal: :Journal of molecular biology 2014
Andrea L Edwards Tsutomu Matsui Thomas M Weiss Chaitan Khosla

The 6-deoxyerythronolide B synthase (DEBS) is a prototypical assembly line polyketide synthase produced by the actinomycete Saccharopolyspora erythraea that synthesizes the macrocyclic core of the antibiotic erythromycin 6-deoxyerythronolide B. The megasynthase is a 2-MDa trimeric complex composed of three unique homodimers assembled from the gene products DEBS1, DEBS2, and DEBS3, which are hou...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1999
R McDaniel A Thamchaipenet C Gustafsson H Fu M Betlach G Ashley

The structures of complex polyketide natural products, such as erythromycin, are programmed by multifunctional polyketide synthases (PKSs) that contain modular arrangements of functional domains. The colinearity between the activities of modular PKS domains and structure of the polyketide product portends the generation of novel organic compounds-"unnatural" natural products-by genetic manipula...

2016
Birgit Uytterhoeven Kenny Appermans Lijiang Song Joleen Masschelein Thomas Lathouwers Chris W. Michiels Rob Lavigne

Kalimantacin is an antimicrobial compound with strong antistaphylococcal activity that is produced by a hybrid trans-acyltransferase polyketide synthase/nonribosomal peptide synthetase system in Pseudomonas fluorescens BCCM_ID9359. We here present a systematic analysis of the substrate specificity of the glycine-incorporating adenylation domain from the kalimantacin biosynthetic assembly line b...

Journal: :Bioresources and Bioprocessing 2021

Abstract Background Neoantimycins are a group of 15-membered ring depsipeptides isolated from Streptomycetes with broad-spectrum anticancer activities. Neoantimycin biosynthesis is directed by the hybrid multimodular megaenzymes non-ribosomal peptide synthetase and polyketide synthase. We previously discovered new neoantimycin analogue unantimycin B, which was demonstrated to have selective act...

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