Designing of controlled-release drug delivery systems is a promising and rapidly developing line of pharmacology. The main advantage of using drug delivery systems is that drug concentration in a patient’s blood and/or tissues can be maintained at a target level for an extended time (Kost 2001). The principal requirement for fabricating prolonged-action controlled-release drug delivery systems ...

BACKGROUND Most bacteria are grown in a binary fission way meaning a bacterial cell is equally divided into two. Polyhydroxyalkanoates (PHA) can be accumulated as inclusion bodies by bacteria. The cell division way and morphology have been shown to play an important role in regulating the bacterial growth and PHA storages. RESULTS The common growth pattern of Escherichia coli was changed to m...

Transformation of Escherichia coli plays an important role in recombinant DNA technology. Most current transformation protocols require that the cells be treated to attain a particular physiological state known as "competence," and this makes transformation procedures lengthy and arduous. Here we describe a protocol for transforming log-phase E. coli using dimethyl sulfoxide (DMSO) solutions of...

Biocomposites is a field that has gained much scientific attention due to the current substantial consumption of nonrenewable resources and the environmentally harmful disposal methods required for traditional polymer composites. Research on natural fiber reinforced polyhydroxyalkanoates (PHAs) has gained considerable momentum over the past decade. There is little work on PHAs reinforced with u...

Application of polyhydroxybutyrate (PHB) to plastic industry has expanded over the last decades due to its attracting features over petro-based plastic, and therefore, its waste accumulation in nature is inevitable. In the present study, a total of four bacterial strains, viz., MK3, PN12, PW1, and Lna3, were formulated into a consortium and subsequently used as biological tool for degradation o...

Establishing industrial biotechnology for the production of chemical compounds from the biosynthetic pathway has received a significant boost with the implementation of metabolic engineering. At present, metabolic engineering in Saccharomyces cerevisiae gains significant advantages of integration of knowledge acquired through a long history of use and data acquisition from novel –omics technolo...