The goal of this research is to optimize PHA production in the syngas fermenting organism Rhodospirillum rubrum. Syngas fermentation is the hybrid thermochemical/ biological approach to processing biomass into valuable fuels and chemicals. The process begins with the gasification of biomass to produce syngas, a flammable gas mixture consisting primarily of carbon monoxide (CO), hydrogen (H2), a...

The recovery of biopolymers from sewage sludge could be a crucial step in implementing circular economy principles wastewater treatment plants (WWTP). In this frame, the present study was aimed at evaluating simultaneous production polyhydroxyalkanoates (PHA) and extracellular polymeric substances (EPS) obtainable agro-industrial wastewater. Two biological enrichment systems, aerobic granular (...

Seven strains of sulfate-reducing bacteria (SRB) were tested for the accumulation of polyhydroxyalkanoates (PHAs). During growth with benzoate Desulfonema magnum accumulated large amounts of poly(3-hydroxybutyrate) [poly(3HB)]. Desulfosarcina variabilis (during growth with benzoate), Desulfobotulus sapovorans (during growth with caproate), and Desulfobacterium autotrophicum (during growth with ...

Polyphosphate accumulating organisms (PAOs) belong mostly to Proteobacteria and Actinobacteria and are quite divergent. Under aerobic conditions, they accumulate intracellular polyphosphate (polyP), while they typically synthesize polyhydroxyalkanoates (PHAs) under anaerobic conditions. Many ecological, physiological, and genomic analyses have been performed with proteobacterial PAOs, but few w...

The process for the production of biodegradable plastic material (polyhydroxyalkanoates, PHAs) from microbial cells by mixed-bacterial cultivation using readily available waste (renewable resources) is the main consideration nowadays. These observations have shown impressive results typically under high carbon fraction, COD/N and COD/P (usually described as nutrient-limiting conditions) and war...

BACKGROUND Polyhydroxyalkanoates (PHA) are synthesized by many bacteria in the cytoplasm as storage compounds for energy and carbon. The key enzymes for PHA biosynthesis are PHA polymerases, which catalyze the covalent linkage of 3-hydroxyacyl coenzymeA thioesters by transesterification with concomitant release of CoA. Pseudomonas putida GPo1 and many other Pseudomonas species contain two diffe...

The aims of these experiments were treating tapioca processing waste water to produce polyhydroxyalkanoates (PHAs) and removal of chemical oxygen demand (COD). The experiments were conducted in a sequencing batch reactor with a volume of 6 L. The entire experimental condition was anaerobic-aerobic-anaerobic. Experiment 1, 2 and 3 had the first 2 h for feeding time while Experiment 4 had 3 h. Th...

Saline Mussels Cooking Wastewater was valorised to produce PHA with Mixed Microbial Cultures (MMC). Due the high protein content (1.8–5.7 g CODPROT/L), accumulating capacity below 10%, so several strategies were tested. In acidification unit, Na(HCO3) added, increasing conversion into Volatile Fatty Acids (VFA) from 10.3% 69.2% and subsequent accumulation 6.9 14.7%. enrichment incorporation of ...

Polyhydroxyalkanoates (PHAs) are a group of bioplastics that have a wide range of applications. Extensive progress has been made in our understanding of PHAs' biosynthesis, and currently, it is possible to engineer bacterial strains to produce PHAs with desired properties. The substrates for the fermentative production of PHAs are primarily derived from food-based carbon sources, raising concer...

Sugarcane bagasse (SCB), one of the major lignocellulosic agro-industrial waste products, was used as a substrate for biosynthesis of polyhydroxyalkanoates (PHA) by halophilic archaea. Among the various wild-type halophilic archaeal strains screened, Halogeometricum borinquense strain E3 showed better growth and PHA accumulation as compared to Haloferaxvolcanii strain BBK2, Haloarcula japonica ...