One fraction designated FV-7, in the water soluble ingredient if the pollen extract Cernilton was found to be inhibitory to the growth of a prostate cancer cell line. Characterization of FV-7 by high-resolution mass spectrometry and nuclear magnetic resonance identified as hydroxamic acid, 2,4-dihydroxy-2H-1, 4benzoxazin-3 (4H)-one (DIBOA). To confirm this further, we synthesized an authentic s...

A free radical-scavenging preparation (pollen extract) or the corresponding placebo was given to 50 volunteers over a period of 4 weeks to test the hypothesis that muscle soreness is associated with the generation of free radicals. The increase in malonyldialdehyde and lactate immediately after exercise both in blood and in muscle tissue was significantly lower after treatment with the scavengi...

We reviewed published data concerning the ability of a defined flower pollen ex-tract derived from rye, corn, and timothy, commonly referred to as PollenAid to provide symptomatic relief in men suffering from lower urinary tract symptoms (LUTS). This same defined pollen extract has also been called PollenAid TM , Cernilton, and Prostaphil in other reports and is commercially available as Gramin...

the allergoid (P 1⁄4 0.16; Mann-Whitney). Linear regression coefficients obtained between immunoglobulin reactivity against both extracts were: r2 1⁄4 0.51 for specific IgE and r2 1⁄4 0.83 for specific IgG. An important decrease in the allergenic activity, measured by inhibition ELISA, was clearly observed. The MS/MS assay revealed the presence of the mayor allergen, and some isoforms, in non-m...

BACKGROUND Various studies have shown the clinical efficacy of sublingual immunotherapy in grass pollen-induced rhinoconjunctivitis. However, even short-term treatment with grass extracts might cause sensitizations to formerly unrecognized antigens. OBJECTIVE To determine whether the antibody profiles are changing in patients receiving a defined grass pollen extract prior to and during the gr...

the allergoid (P 1⁄4 0.16; Mann-Whitney). Linear regression coefficients obtained between immunoglobulin reactivity against both extracts were: r2 1⁄4 0.51 for specific IgE and r2 1⁄4 0.83 for specific IgG. An important decrease in the allergenic activity, measured by inhibition ELISA, was clearly observed. The MS/MS assay revealed the presence of the mayor allergen, and some isoforms, in non-m...

Evaluation of the pistillate flower abscission (PFA) potential of UC-67-13 and UC-67-11 was continued at two sites. At a high PFA site UC-67-13 PFA was only moderately lower than Serr for the third year. UC-67-11 demonstrated low PFA potential for a seconq year at this site. Higher PFA potential of UC-67-13 than UC-67 -11 was also found at a second site for a second year. No further evaluation ...

The influence of a standardized pollen extract (Cernitin) on the hepatic cytochrome P-450 system was studied in rats chronically exposed to an aerosol of ammonium fluoride. The content of cytochrome P-450 and cytochrome b5 and the activities of NADPH-cytochrome c reductase, aminophenazone N-demethylase, and aniline hydroxylase were determined. After 3 months the content of cytochrome b5 in the ...

A vast array of actin binding proteins (ABPs), together with intracellular signaling molecules, modulates the spatiotemporal distribution of actin filaments in eukaryotic cells. To investigate the complex regulation of actin organization in plant cells, we designed experiments to reconstitute actin-ABP interactions in vitro with purified components. Because vertebrate skeletal [alpha]-actin has...

Thymocytes must pass a series of checkpoints on the road to becoming mature T cells. On page 603, Mandal et al. show that cells use Bcl2A1, a member of the Bcl2 family of survival proteins, to pass one critical checkpoint. The cell cannot make substitutes for this protein in a pinch, revealing that Bcl2A1 may be uniquely specialized for promoting survival without suppressing proliferation. To b...