The wavelength dependence of the incoherent point spread function in a wide-field microscope was investigated experimentally. Dispersion in the sample and optics can lead to significant changes in the point spread function as wavelength is varied over the range commonly used in fluorescence microscopy. For a given sample, optical conditions can generally be optimized to produce a point spread f...

We present a virtual image refocusing method over an extended depth of field (DOF) enabled by cascaded neural networks and double-helix point-spread function (DH-PSF). This network model, referred to as W-Net, is composed two generator discriminator pairs. The first learns virtually refocus input onto user-defined plane, while the second perform cross-modality transformation, improving lateral ...