Porcine circovirus type 2 (PCV2) is the primary causative agent of postweaning multisystemic wasting syndrome (PMWS) in pigs. To identify potential genetic determinants for virulence and replication, we serially passaged a PCV2 isolate 120 times in PK-15 cells. The viruses harvested at virus passages 1 (VP1) and 120 (VP120) were biologically, genetically, and experimentally characterized. The P...

BACKGROUND The systemic form of porcine circovirus associated disease (PCVAD), also known as postweaning multisystemic wasting syndrome (PMWS) was initially detected in the early 1990s. Starting in 2004, the Canadian swine industry experienced considerable losses due to PCVAD, concurrent with a shift in genotype of porcine circovirus type 2 (PCV2). Objectives of the current study were to explor...

not available 687 Krakowka, S., Ringler, S.S., Arumugam, P. , McKillen, J., McIntosh, K., Hartunian, C., Hamberg, A., Rings, M., Allan, G., Ellis, J.A. (2008) Evaluation of Mycoplasma hyopneumoniae bacterins for porcine torque teno virus DNAs American Journal of Veterinary Research, 69, 1601-1607 Objective-TO determine whether commercial Mycoplasma hyopneumoniae bacterins sold for use in swine ...

Sets of oligonucleotide primers were designed according to the sequences of the open reading frames (ORFs) ORF1 and ORF2 of the prototype nonpathogenic PK-15 strain of porcine circovirus (PCV) type 1 (PCV-1). By the PCR performed with the various primer sets, genomic DNA or RNA from other bacterial or viral pathogens of the respiratory tracts of pigs could not be amplified. A positive amplifica...

Porcine circovirus type 2 (PCV2), an economically important pathogen, causes postweaning multisystemic wasting syndrome (PMWS) and other syndrome diseases collectively known as porcine circovirus-associated disease (PCVAD). Previous studies revealed breed-dependent differences in porcine susceptibility to PCV2; however, the genetic mechanism underlying different resistance to PCV2 infection rem...

Recently, studies have suggested an association between the Torque teno sus virus (TTSuV) and the Porcine circovirus-2 (PCV2) in PCV2-associated disease cases. The aim of this study was to verify TTSuVs loads in pig faeces from PCV2-positive animals with and without diarrhea from PCVADaffected and PCV2-unvaccinated herds. A total of 80 faecal samples were collected individually from nursery and...

We report on establishing the polydispersity in chemical composition (PCC) and polydispersity in monomer sequence distribution (PMSD) in random copolymers of poly(styrene-co-4-bromostyrene) (PBrxS), where x = (0.385 ± 0.035) is the mole fraction of the 4bromostyrene units (4-BrS), prepared by electrophilic substitution of bromine in the para-position of the phenyl ring of the parent polystyrene...