Background: Numerous studies have been conducted on antimicrobial properties that may be useful to overcome the emerging resistance. Plumbago zeylanica (Ela nitul) is especially known as Ceylon leadwort. has used in traditional medicine treat various diseases, including skin rashes, scabies, ringworm, hookworms, dermatitis, acne, sores, and ulcers. Objectives: The objective of this study was ac...

catharanthus roseus is a medicinal and ornamental plant with growing attention toward its economical value. cell suspension from c. roseus were treated with 0 to 60mm of cadmium nitrate for 1, 3 and 6 days, then cell viability was determined using trypan blue and mtt assay. cell morphology was investigated using 0, 10, 30 and 50 mm of cadmium nitrate as selected dose for 3 days. callus of c.ros...

Dasamularista was prepared as per the national formulary to study the effect of container on the production of alcohol and the presence of microorganisms during the process of fermentation. Citraka (Plumbago rosea Linn.) which was one of the ingredient in the drug and subjected to "Sodhana" was also studied. The Citraka was impoverished by 50% with respect to plumbagin as a result of sodhana-pu...

A reverse phase HPLC method with UV detection has been developed and validated in order to quantify plumbagin, the bioactive marker of the roots of P. indica and P. zeylanica. A quantitative HPTLC method was also developed using hexane: ethyl acetate (8:2) as the mobile phase. The plumbagin content in the roots were determined using both the methods. P. indica was found to contain significantly...

Catharanthus roseus is a medicinal and ornamental plant with growing attention toward its economical value. Cell suspension from C. roseus were treated with 0 to 60mM of cadmium nitrate for 1, 3 and 6 days, then cell viability was determined using trypan blue and MTT assay. Cell morphology was investigated using 0, 10, 30 and 50 mM of cadmium nitrate as selected dose for 3 days. Callus of C.ros...

A protocol is described for individually collecting two populations of sperm cells, Svn and Sua, from pollen of Plumbago zeylanica. Pollen grains were burst in 10 mM MOPS buffer containing 0.8 M mannitol (pH 4.6). Paired sperm cells released from pollen were separated using a microinjector. Svn and Sua were then collected individually with a microinjector, based upon known size differences. Col...