Triolein/cholesteryl oleate/cholesterol/phosphatidylcholine emulsions designed to model the lipid composition of chylomicrons were injected intravenously into control and streptozotocin-treated insulin-deficient rats. As previously described for lymph chylomicrons, the emulsion triolein was hydrolysed and phosphatidylcholine was transferred to the plasma high-density lipoproteins (HDL). This me...

BACKGROUND There is considerable evidence that remnants of triglyceride-rich lipoproteins may be particularly atherogenic. METHODS AND RESULTS Levels of lipoprotein lipids and of apolipoprotein B in low-density lipoproteins were measured in 335 men and women enrolled in a study in which quantitative coronary angiography was carried out at 2-year intervals. Clinical events related to coronary ...

Introduction: Nanoparticle tracking analysis (NTA) enables measurement of extracellular vesicles (EVs) but lacks the ability to distinct between EVs and lipoproteins which are abundantly present in blood plasma. Limitations in ultracentrifugation and size exclusion chromatography applied for EV isolation may result in inadequate EV purification and preservation. In this proof of concept study, ...

The immunochemical technique, first employed in the study of albumin synthesis in chicken liver slices by Peters and Anfmsen (3), has recently been successfully used with rat liver slices by Marsh and Drabkin (4) as well as by Campbell and Stone (5). The present work extends this technique to the problem of the biosynthesis of plasma lipoprotein by rat liver. The liver has been shown to be the ...

Triglyceride-rich lipoprotein apoprotein catabolism was studied in rats from 5 to 60 min after intravenous injection of 125I-labeled lipoproteins. The plasma and liver labeled apoprotein content was analyzed by gel filtration column chromatography using an elution buffer containing 1% sodium dodecyl sulfate. The method resolved two B apoproteins of lower (apo B1) and higher (apo Bh) molecular w...

Structural lipids, primarily phospholipids and sterols, as well as energy-storage lipids such as triacylglycerols are insoluble in water. In the aqueous fluids of animals, they are carried as lipoproteins, complexes of lipids bound to polypeptides. These watersoluble lipoproteins can be separated into different classes, depending upon their hydrated densities. These classes include very low den...