The release and survival of ascospores of a UK Sclerotinia sclerotiorum isolate were studied. Apothecia placed in a spore clock apparatus with different lighting regimes at 15 degrees C released ascospores continuously with an increasing rate for the duration of experiments (72-84 h). Spore release was not confined to light or dark periods in alternating regimes and occurred in continuous dark ...

Oxalic acid is an important virulence factor for disease caused by the fungal necrotrophic pathogen Sclerotinia sclerotiorum, yet calcium oxalate (CaOx) crystals have not been widely reported. B. carinata stems were infected with S. sclerotiorum and observed using light microscopy. Six hours post inoculation (hpi), CaOx crystals were evident on 46% of stem sections and by 72 hpi on 100%, demons...

Resistance of benzimidazole fungicides is related to the point mutation of the β-tubulin gene in Sclerotinia sclerotiorum. The point mutation at codon 198 (GAG → GCG, E198A) occurs in more than 90% of field resistant populations in China. Traditional detection methods of benzimidazole-resistant mutants of S. sclerotiorum are time-consuming, tedious and inefficient. To establish a suitable and r...

AIMS To establish the modes of action of the antagonistic fungal strain Clonostachys rosea BAFC3874 isolated from suppressive soils against Sclerotinia sclerotiorum and to determine its potential as a biocontrol agent. METHODS AND RESULTS The antagonistic activity of C. rosea BAFC3874 was determined in vitro by dual cultures. The strain effectively antagonized S. sclerotiorum in pot-grown let...

Sclerotinia stem rot (SSR) is one of the most destructive disease mustard which affects quality and quantity crop resulting in severe yield losses. The present study was conducted to evaluate bio-efficacy different Trichoderma spp. plant extracts against sclerotiorum under laboratory conditions. All tested showed a significant effect on growth inhibition test fungus over control. Among spp., ma...

backgoround: plant diseases, caused by a wide range of phytopathogenic fungi, could be managed using of trichoderma sp, as a biocontrol agent. cell wall degrading enzymes like chitinase from t. harzianum are important means for fungal pathogen inhibition. overexpression of these chitinase enzymes can improve the antagonistic potential of trichoderma sp. strains. objectives: this study aimed to ...

Cecile Lahiri, Emory University Katherine B. Dugan, Emory University Xianhong Xie, Albert Einstein College of Medicine Laura Reimers, Albert Einstein College of Medicine Robert D. Burk, Albert Einstein College of Medicine Kathryn Anastos, Albert Einstein College of Medicine Stewart Massad, Washington University Isam-Eldin Eltoum, University of Alabama at Birmingham Xiaonan Xue, Albert Einstein ...

Jingjing ZHANG, Yuxin LIN, Guojun DAI*, Tingting AN, Mingming SUN, Genxi ZHANG, Kaizhou XIE, Jingyu WANG College of Animal Science and Technology, Yangzhou University, Yangzhou, P.R. China Key Laboratory for Animal Genetics, Breeding and Reproduction and Molecular Design of Jiangsu Province, Yangzhou, P.R. China Department of Animal Science, College of Agriculture and Natural Resources, Univers...

Macrophomina phaseolina and Sclerotinia sclerotiorum are two significant fungal pathogens of sunflower. M. causes charcoal rot ashy stem blight in several important crop species. white mold disease which can occur as middle stalk rot, head premature plant death. Due to the wide host range their survival structures, rotation cannot provide sufficient protection against them. In our experiment, w...

Pseudomonas sp. DF41 is a biocontrol agent capable of suppressing Sclerotinia sclerotiorum-mediated stem rot of canola. Using transposon mutagenesis, we identified two mutants with greatly reduced antifungal (AF) activity. The first mutant had an insertion in gacS, forming part of the GacS/GacA regulatory system. The second mutation was in a gene involved in lipopeptide (LP) synthesis suggestin...