This study evaluated the influence of the manipulation technique and polishing method on the flexural strength and cytotoxicity of acrylic resins. Two manipulation techniques and three polishing methods were used in the fabrication of acrylic plates that were divided into 6 groups (n=10). Groups MM, MC and MW: mass technique with mechanical polishing, chemical polishing and without polishing, r...

INTRODUCTION In 1902 Gottleib Haberlandt proposed the idea of growing individual plant cells on artificial medium. While Haberlandt never realized his idea, the 105 years since has seen the concept evolve into a powerful tool utilized throughout the plant sciences. Plant tissue culture broadly refers to growing plant cells, tissues, organs, seeds, or other plant parts in a sterile environment o...

Background and objectives: The seeds of Securigera securidaca (L.) Deg. & Dorf. (Fabaceae) are used as anti-diabetic remedy in Iranian folk medicine. The aim of the present study was to establish the callus and suspension culture of S. securidaca seeds for the first time and to determine the major secondary metabolites including cardiac glycosides and total ph...

      Ginger plant, Zingiber officinale Roscoe, is an important tropical plant used as spices and well known for its medicinal properties. It has a pungent and aromatic rhizome rich of zingiberen and gingerols. Many secondary metabolites are known to accumulate in the plant cell culture systems. So, in this research, gingerols and zingiberene production of callus cultures were compared...

Lithospermum erythrorhizon cell cultures have been used to produce plant secondary metabolites, as well as in biosynthetic studies. Shikonin, a representative secondary metabolite of L. erythrorhizon, was first produced industrially by dedifferentiated cell cultures in the 1980s. This culture system has since been used in research on various plant secondary metabolites. Other boraginaceaeous pl...

BACKGROUND Medicinal plants are becoming more popular in the treatment of various diseases because of the adverse effects of the current therapy, especially antioxidant plant components such as phenols and flavonoids have a protective role against oxidative stress-induced degenerative diseases like diabetes. Thus, the purpose of this study was to investigate β-cell protection and antidiabetic a...

BACKGROUND Human cholinesterases can be used as a bioscavenger of organophosphate toxins used as pesticides and chemical warfare nerve agents. The practicality of this approach depends on the availability of the human enzymes, but because of inherent supply and regulatory constraints, a suitable production system is yet to be identified. RESULTS As a promising alternative, we report the creat...

Mammalian cell culture media used for the manufacture of therapeutic protein have advanced systematically from serum-containing into animal-free, protein-free and chemically defined formulations over the past decades. Initially driven by patient safety concerns associated with the use of animal-derived medium components, and later by inconsistent cell culture performance due to variability in p...