High fluorodeoxyglucose (FDG) uptake by human carcinomas, including head and neck cancers, is associated with a poor prognosis. Glucose transporter-1 (Glut-1) is believed to be an intrinsic marker of hypoxia in malignant tumors. The expression of hypoxia-inducible factor-1α (HIF-1α) and correlated target genes, including Glut-1, is regulated by the phosphoinositide 3-kinase/protein kinase B (PI...

Direct genetic alterations leading to deregulated PI3K/Akt signaling are common in a significant fraction of human malignancies. Both Akt and Aurora A kinase have been shown to be important targets for intervention for cancer therapy. Nuclear Akt1 expression and Akt activation are common in cancer invasion. However, the mechanisms for this association and its causal role in invasion are uncerta...

BACKGROUND Zuo-Jin-Wan (ZJW), a traditional Chinese medicine formula, has been identified to be effective against drug resistance in cancer. In the present study, we investigated the effect of ZJW on acquired oxaliplatin-resistant and the PI3K/Akt/NF-κB pathway in vitro. METHODS We tested the dose-response relationship of ZJW on reversing drug-resistance by CCK-8 assay and flow cytometry anal...

T-cell large granular lymphocytic leukemia (T-LGL) is characterized by chronic clonal lymphoproliferation of cytotoxic T lymphocytes (CTLs). Despite exhibiting phenotypic properties of antigen-activated cells, including expression of Fas and FasL, T-LGL cells accumulate and demonstrate resistance to apoptosis. We propose that increased activity of a prosurvival signaling pathway in T-LGL is res...

Cellular signalling pathways are critical in regulating the balance between latency and lytic replication of herpesviruses. Here, we investigated the effect of the phosphatidylinositol 3-kinase (PI3K)-Akt pathway on replication of two gamma-2 herpesviruses, murine gammaherpesvirus-68 (MHV-68) and human herpesvirus-8/Kaposi's sarcoma-associated herpesvirus (HHV-8/KSHV). We found that de novo inf...

Successful implantation requires endometrial receptivity. To investigate the mechanisms of miR-494-3p on receptivity, GnRHa's superovulation scheme was designed to reduce and pregnant mice were injected with antagomir. The regulatory role identified by RT-qPCR, uterine blastocyst count, scanning electron microscopy, hematoxylin-eosin (HE) staining, Western blot. Results indicated that antagomir...

Identification of the phosphoinositide-3-kinase–protein kinase B/Akt (PI3K-PKB/Akt) pathway and activating receptor tyrosine kinases (RTKs) began in earnest in the early 1980s through vigorous attempts to characterize insulin receptor signaling (for review, see Alessi 2001; Brazil and Hemmings 2001). These humble beginnings led to the identification of the components and mechanism of insulin re...

OBJECTIVE Integrins mediate platelet adhesion and transmit outside-in signals leading to platelet spreading. Phosphoinositide 3-kinases (PI3Ks) play a critical role in outside-in signaling and platelet spreading; however, the mechanisms of PI3K activation and function in outside-in signaling are unclear. We sought to determine the role of the Akt family of serine/threonine kinases and activatio...

Ionizing radiation induces an intracellular stress response via activation of the phosphatidylinositol 3'-kinase (PI3K)/Akt survival pathway. In tumor cells, the PI3K/Akt pathway is induced through activation of members of ErbB receptor tyrosine kinases. Here, we investigated the receptor dependence of radiation-induced PI3K/Akt activation in tumor cells and in endothelial cells. The integrity ...

objective(s): study of non-coding rnas is considerable to elucidate principal biological questions or design new therapeutic strategies. mirnas are a group of non-coding rnas that their functions in pi3k/akt signaling and apoptosis pathways after t cell activation is not entirely clear. herein, mirnas expression and their putative targets in the mentioned pathways were studied in the activated ...