In 1939 Blnuch and Koch (1) published an enzymatic method for the calorimetric determination of uric acid in whole blood. This method involves the use of the enzyme uricase for the specific destruction of uric acid. “True uric acid” values were obtained by the difference in the intensity of color produced before and after incubation with the enzyme. The authors reported “true uric acid” values ...

In earlier publications (Keyser, 1949, 1950) we reported increases in the level of a substance giving the tryptophane-perchloric acid reaction (Cohen, 1944; Seibert, Pfaff, and Seibert, 1948) in the blood serum of six burn cases. No attempt was made at the time to identify the reacting substance, which is generally assumed to be a polysaccharide. The present investigation was undertaken to find...

TANRET [1909] isolated from ergot of rye a base which he termed ergothioneine, which was shown by Barger and Ewins [1911] to be the betaine of thiolhistidine. There is no further record of the occurrence of ergothioneine in the plant or animal kingdom until the isolation of a substance from blood by Hunter and Eagles [1925, 1927], which has recently been shown by Eagles and Johnson [1927] to be...

N-methyl imidazolium phosphotungstic salt has been synthesized and used as a special catalyst for photopolymerization of vinyl monomers. This is a fast and smooth reaction, and high molecular weight polymers with narrow polydispersity are obtained within 60 min. The compound was structurally characterized by elemental analysis, IR spectroscopy, and 1H NMR spectroscopy. The electrochemical prope...

The Lowry protein assay is widely used to quantify proteins in the laboratory. As the assay is susceptible various interfering substances, the protein test sample is frequently first purified by precipitating the proteins using trichloroacetic acid (TCA). However, TCA precipitates proteins poorly in the presence of sodium dodecyl sulfate (SDS), a detergent very commonly used in biological resea...