Label claims of vitamin E succinate and polyphenolic nutraceuticals are assessed. A validated HPLC method was utilized to assess vitamin E succinate products. Three novel LC/MS methods were used to assess the polyphenols, pterostilbene, phloretin, and myricetin, in dietary supplements. The amount of vitamin E succinate varied from 0-130% of the stated label content with two products containing ...

Reaction with alcohol. The glycosidic OH group of mutarotating sugars react with alcohols to form αand βglycosides or acetals. Thus, glucose forms glucosides and fructose forms fructosides. The glucosides, or glycosides in general, do not exhibit mutarotation as the aldehyde group in them is converted to the acetal group. A number of glycosides occur in nature, e.g., phlorhizin (glucose + phlor...

Ethanol extracts of Piper aduncum L. leaves from five sub-regions in the tropical Peruvian rainforest were assessed for polyphenol content utilizing liquid chromatography coupled with mass spectrometry with electrospray ionization (LC-MS-ESI). The anti-oxidant capacity was estimated using a DPPH radical scavenging assay. Additionally, the reducing power (capacity to reduce Fe 3+ to Fe 2+ ), the...

Adipocytes were isolated from epididymal fat pads from the same rats by the method of Rodbell [4] and resuspended in Krebs bicarbonate buffer containing 4% (w/v) bovine serum albumin and 0.1 mM-D-glucose. Aliquots of cell suspension were preincubated for either IS min (myocytes) or 10 min (adipocytes) with or without insulin. Transport was started by the addition of [3H]-2deoxy-11-glucose and s...

The mature, intraerythrocytic form of the human malaria parasite, Plasmodium falciparum, is reliant on glycolysis for its energetic requirements. It produces large quantities of lactic acid, which have to be removed from the parasite's cytosol to maintain the cell's integrity and metabolic viability. Here we show that the monocarboxylates lactate and pyruvate are both transported across the par...

Purpose: To study the metabolites of phloretin in vivo using ultra-high performance liquid chromatography linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap).
 Methods: After administration (50 mg/kg; oral route) to six rats, blood samples were taken from each animal. Each sample was then subjected solid-phase extraction prepare it for chromatographic/spectroscopic analysis. Fi...

The aim of this study was to compare total phenolic content (TPC), radical-scavenging activity (RSA), anthocyanin (TAC), sugar and polyphenolic profiles two apple cultivars (‘Discovery’ ‘Red Aroma Orelind’) from organic integrated production systems in climatic conditions Western Norway. Sixteen sugars four alcohols 19 polyphenols were found the peel, but less detected pulp. peel both apples ha...

Phloridzin (phlorizin or phloretin 2'-O-glucoside) is known for blocking intestinal glucose absorption. We have investigated the anticarcinogenic effect of phloridzin and its novel derivatives using human cancer cell lines. We have synthesised novel acylated derivatives of phloridzin with six different long chain fatty acids by regioselective enzymatic acylation using Candida Antarctica lipase ...

Capillaries were isolated from bovine retina by homogenization and glass bead filtration in order to study their ability to transport certain solutes. Viability of the microvessels was demonstrated by their ability to maintain linear rates of substrate oxidation for more than two hours. He.uose uptake (measured using 3-o-methyl-D-glucose) could be inhibited b3 cytochalasin R, phloretin and phlo...