The mRNAs encoding two enzymes of phenylpropanoid metabolism, phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) and 4-coumarate:CoA ligase (4CL; EC 6.2.1.12), were induced in cultured parsley cells (Petroselinum hortense) either by irradiation with UV light or by treatment with elicitor, a cell-wall fraction of the fungus Phytophthora megasperma f. sp. glycinea. Two-dimensional gel electrophoresis ...

The time course and spatial distribution of wound-induced activities of phenylalanine ammonia-lyase and peroxidase were determined to establish correlations between molecular and cellular aspects of the wound-induced pattern of cell differentiation in potato (Solanum tuberosum L.) tissue. A high correlation between peroxidase activity and suberization was observed. Peroxidase activity increased...

Potato (solanum tuberosum L. cv Katahdin) tuber discs treated with arachidonic acid become necrotic and accumulate sesquiterpenoid phytoalexins. The arachidonic acid also causes increases in both phenylalanine ammonia lyase and lignin, but no change in total alcohol-soluble phenols. Linoleic acid does not alter any of these parameters. A high concentration of nonanoic acid promotes both necrosi...

The plant elicitors use as an agricultural input is limited and few studies show their influence on physiological responses. However, its can induce broad-spectrum long-lasting resistance in plants by inducing defense systems, primary secondary metabolism. the aim of this study was to evaluate gas exchange (gs, A, E, Ci, WUE, iWUE iCE) enzymatic antioxidant system (POD, PPO PAL) Z. mays under a...

Activation of plant defense genes was investigated by analysis of transcripts completed in vitro by isolated nuclei. Elicitor treatment of suspension-cultured bean (Phaseolus vulgaris L.) cells caused marked transient stimulation of transcription of genes encoding apoproteins of cell wall hydroxyproline-rich glycoproteins (HRGP) and the phenylpropanoid biosynthetic enzymes phenylalanine ammonia...

We developed a model system to study the signal transduction pathways leading to the activation of Arabidopsis thaliana genes involved in the defense against pathogen attack. Here we describe the identification and characterization of virulent and avirulent Pseudomonas syringae strains that elicit disease or resistance symptoms when infiltrated into Arabidopsis leaves. The virulent and avirulen...

Phenylpropanoid biosynthesis in plants engenders myriad phenolics with diverse biological functions. Phenylalanine ammonia-lyase (PAL) is the first committed enzyme in the pathway, directing primary metabolic flux into a phenylpropanoid branch. Previously, we demonstrated that the Arabidopsis (Arabidopsis thaliana) Kelch domain-containing F-box proteins, AtKFB01, AtKFB20, and AtKFB50, function ...

The phenylpropanoid pathway serves as a rich source of metabolites in plants. It is required for the biosynthesis of lignin and acts as a starting point for the production of many other important compounds involved in growth and development. Phenylalanine ammonia-lyase (PAL) catalyzes the first step of the phenylpropanoid pathway. PAL gene expression changes during the growth and development of...

The lack of label-free high-throughput screening technologies presents a major bottleneck in the identification active and selective biocatalysts, with number variants often exceeding capacity traditional analytical platforms to assess their activity practical time scale. Here, we show application direct infusion biotransformations mass spectrometer (DiBT-MS) variety enzymes, different formats,...