Inbred weanling rats were maintained on diets containing low (6%), medium (lS%), and high (40%) levels of proteins. After 45 days they were killed and the activities of histidine ammonia lyase, urocanase, and histidine pyruvate aminotransferase in liver were assayed. Histidine ammonia lyase increased with age of the rat, and the increase was proportional to the level of protein in the diet. Ani...

The effect of water deficit on flavonoid production and physiological parameters characteristic for oxidative stress were studied in a cell suspension culture of Glycyrrhiza inflata Batal to investigate its drought tolerance. The result indicated that appropriate water deficit enhanced biomass accumulation of 27.1 g L(-1) and flavonoid production of 151.5 mg L(-1), which was about 2-fold and 1....

Extra-Virgin Olive Oil (EVOO) is a fundamental component of the Mediterranean diet and it may contain several anti-oxidant substances, such as phenols. Previous research has shown that this food may be enriched in phenols by spraying a sodium-selenate solution (100 mg L-1 Se) onto the crop canopy before flowering. The aim of this research was to evaluate the effect of this Se-fertilization befo...

Inbred weanling rats were maintained on diets containing low (6%), medium (lS%), and high (40%) levels of proteins. After 45 days they were killed and the activities of histidine ammonia lyase, urocanase, and histidine pyruvate aminotransferase in liver were assayed. Histidine ammonia lyase increased with age of the rat, and the increase was proportional to the level of protein in the diet. Ani...

Resistance responses in the leaves of eight rose genotypes, Knock Out (highly resistant), Arka Nishkant (moderately R. multiflora susceptible), Swadesh IIHRR 13-4 (susceptible), Parimala indica (susceptible) and 4-15-12 exhibiting varied levels resistance against black spot were investigated post artificial inoculation with pathogen, Diplocarpon rosae. There was consistent increase activities d...

Aromatic amino acid ammonia-lyases and aromatic amino acid 2,3-aminomutases contain the post-translationally formed prosthetic 3,5-dihydro-4-methylidene-5H-imidazol-5-one (MIO) group. MIO enzymes catalyze the stereoselective synthesis of α- or β-amino acid enantiomers, making these chemical processes environmentally friendly and affordable. Characterization of novel inhibitors enables structura...

Phenylketonuria (PKU) is an autosomal recessive genetic disorder in which mutations in the phenylalanine-4-hydroxylase (PAH) gene result in an inactive enzyme (PAH, EC 1.14.16.1). The effect is an inability to metabolize phenylalanine (Phe), translating into elevated levels of Phe in the bloodstream (hyperphenylalaninemia). If therapy is not implemented at birth, mental retardation can occur. P...

Aryl metabolite biosynthesis was studied in the white rot fungus Bjerkandera adusta cultivated in a liquid medium supplemented with L-phenylalanine. Aromatic compounds were analyzed by gas chromatography-mass spectrometry following addition of labelled precursors ((14)C- and (13)C-labelled L-phenylalanine), which did not interfere with fungal metabolism. The major aromatic compounds identified ...

Stored cut lettuce gradually turns brown on the cut section after several days of storage, because cutting induces phenylalanine ammonia-lyase (PAL) activity, the biosynthesis of polyphenol is promoted, and the polyphenols are oxidized by polyphenol oxidase. In this study, we screened for inhibitors of PAL derived from fermented broths of microbes and from foods and found that a cinnamon extrac...