This work investigates the environmental impacts of three manufacturing processes for a promising biodegradable polymeric material: polyhydroxyalkanoate (PHA). The processes studied are microbial PHA manufacturing using glucose as the carbon substrate, microbial PHA manufacturing using cheese whey as the carbon substrate, and microbial PHA manufacturing using genetically engineered plants. A li...

Immunosuppressive effect of pregnant serum obtained in the second and third trimesters was demonstrated in the PHA-induced homologous lymphocyte stimulation system, where 3H-thymidine incorporation into DNA was measured. Such an inhibitory factor was not present in the first trimester serum. The immunosuppressive principle detected in the second trimester serum was characterized by a potent inh...

The immunoglobulin G (IgG) binding ZZ domain of protein A from Staphylococcus aureus was fused to the N terminus of the polyhydroxyalkanoate (PHA) synthase from Cupriavidus necator. The fusion protein was confirmed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and mediated formation of ZZ domain-displaying PHA granules in recombinant Escherichia coli. The IgG b...

Primary hepatic angiosarcoma (PHA) is an exceedingly rare and aggressive neoplasm of mesenchymal origin. PHA makes a very small portion primary liver tumors conveys poor prognosis. Symptomatology can be vague often mimics hepatocellular carcinoma upon presentation. Diagnosis requires careful immunohistopathologic confirmation. We present case in patient with abdominal pain suspected underlying ...

Polyhydroxyalkanoates (PHAs) are synthesized and assembled as PHA granules that undergo well-regulated formation in many microorganisms. However, this regulation remains unclear in haloarchaea. In this study, we identified a PHA granule-associated regulator (PhaR) that negatively regulates the expression of both its own gene and the granule structural gene phaP in the same operon (phaRP) in Hal...

The use of molecular diagnostic techniques for bioprospecting and microbial diversity study purposes has gained more attention thanks to their functionality, low cost and quick results. In this context, ten degenerate primers were designed for the amplification of polyhydroxyalkanoate synthase (phaC) gene, which is involved in the production of polyhydroxyalkanoate (PHA)-a biodegradable, renewa...

The current problems with decreasing fossile resources and increasing environmental pollution by petrochemical-based plastics have stimulated investigations to find biosynthetic materials which are also biodegradable. Bacterial reserve materials such as polyhydroxyalkanoates (PHA) have been discovered to possess thermoplastic properties and can be synthesized from renewable resources. Poly-beta...

Anaerobic sludge digestion is the main technology for sludge reduction and stabilization prior to sludge disposal. Nevertheless, methane production from anaerobic digestion of waste activated sludge (WAS) is often restricted by the poor biochemical methane potential and slow hydrolysis rate of WAS. This work systematically investigated the effect of PHA levels of WAS on anaerobic methane produc...

INTRODUCTION Progressive hemifacial atrophy (PHA) is a rare disorder characterized by unilateral facial atrophy affecting the skin, subcutaneous tissue, and fat, muscle, and osteocartilagenous structures creating a sunken hemiface appearance.Etiopathogenesis of PHA is poorly understood; no definitive treatment is currently available. CLINICAL FINDINGS We report a 41-year-old woman with PHA wh...