Prostaglandins have stimulative influence on the human uterus and therefore were introduced to medical treatment in reproductive healthcare as labor inductors or abortifacients. The UHPLC-ESI-QqQ-MS/MS method was developed for six prostaglandins: carboprost, cloprostenol, dinoprost (PGF2α), dinoprostone (PGE2), misoprostol sulprostone (substances pregnancy termination) pharmaceutical samples ap...

The aim of this study was to investigate the effect administration half or full dose prostaglandinF2α (PGF2α; Gestavet Prost, d-cloprostenol, HIPRA®) injection in a routine synchronization protocol following theend breeding period including intravaginal sponge (medroxyprogesterone acetate; Espanjovet, andeCG (Oviser 500, on certain fertility parameters ewes.Two hundred thirty-eight Awassi yearl...

Prostanoids, including prostaglandins (PGs) and thromboxane A2 (TXA2), are a family of lipid-derived autacoids that modulate many physiological systems and pathological contexts. Prostanoids are generated by sequential metabolism of arachidonic acid, catalysed by cyclo-oxygenase, to PGH2, which is then converted to PGD2, PGE2, PGF2α, PGI2 and TXA2, catalysed by their specific synthases. Recent ...

ATP-sensitive potassium (KATP) channels are well characterized in cardiac, pancreatic and many other muscle cells. In the present study, functional expression of the KATP channel was examined in non-pregnant murine longitudinal myometrium. Isometric contraction measurements and Western blot were used. KATP channel openers (KCOs), such as pinacidil, cromakalim, diazoxide and nicorandil, inhibite...

The effect of treatment for anestrus in buffaloes with a PGF2α or GnRH injection and vitamin-mineral (Vit-M) supplementation for 1 to 2 months and some factors influencing the treatment effect were studied. In anestrus buffaloes with CL, an injection of PGF2α tended to show higher estrus detection and pregnancy rates within 17 days after treatment than Vit-M supplementation (P<0.10). In those w...

This study aimed to determine the length of estrus period in Aceh cows based on changes characteristics cervical mucus observed using a camera-equipped artificial insemination (AI) endoscope. used twelve healthy and reproductively sound aged 4-6 years. All were synchronized prostaglandin F2α (PGF2α) injected intramuscularly twice, 11 days apart. Estrus detection was conducted 24 hours following...

Experiment 1 evaluated pregnancy rates (PRs) when estradiol cypionate (ECP) replaced the second GnRH injection of a timed artificial insemination (TAI) protocol for lactating dairy cows. Cows were pre-synchronized with two injections of PGF2α (Lutalyse r , Pharmacia Corp., 25 mg; i.m.) given to multiparous cows (n=170) at 40 ± 3 and 54 ± 3 d postpartum (dpp) and to primiparous cows (n=201) at 6...

Background: Endometrial cancer is the most common gynaecological malignancy; risk factors include exposure to oestrogens and high body mass index. Expression of enzymes involved in biosynthesis of oestrogens and prostaglandins (PG) is often higher in endometrial cancers when compared with levels detected in normal endometrium. Oestrogens bind one of two receptors (ERα and ERβ) encoded by separa...