Soluble expression of recombinant proteins in E. coli is often done by translocation the product across inner membrane (IM) into periplasm, where it retained outer (OM). While integrity IM strongly coupled to viability and impurity release, a decrease OM (corresponding increased “leakiness”) leads accumulation extracellular space, impacting downstream process. Whether leakiness desired or not, ...

The anaerobic bacterium Chrysiogenes arsenatis respires using the oxyanion arsenate (AsO43–) as terminal electron acceptor, where it is reduced to arsenite (AsO33–) while concomitantly oxidizing various organic (e.g., acetate) donors. This respiratory activity catalyzed in periplasm of by enzyme reductase (Arr), with expression controlled a sensor histidine kinase (ArrS) and periplasmic-binding...

Carbohydrate polymers are industrially and medically important. For instance, a polysaccharide, alginate (from seaweed), is widely used in food, textile and pharmaceutical industries. Certain bacteria also produce alginate through membrane spanning multi-protein complexes. Using Pseudomonas aeruginosa as a model organism, we investigated the biological function of an alginate degrading enzyme, ...